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Myc tag Rabbit Polyclonal Antibody

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Catalog# R1208-1

Myc tag Rabbit Polyclonal Antibody

Application

  • WB

  • IP

  • IF-Cell

  • FC

Reactivity

  • Species independent

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

Myc tag Rabbit Polyclonal Antibody

抗体类型

Rabbit Polyclonal Antibody

免疫原

Synthetic peptide within human Myc aa 410-420.

种属反应性

Species independent

验证应用

WB, IP, IF-Cell, FC

阳性对照

PG-CM cell lysates, C-terminal Myc-tagged recombinant protein, N-terminal Myc-tagged recombinant protein.

偶联

unconjugated

RRID

AB_3073204

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Immunogen affinity purified.

应用稀释度

  • WB

  • 1:1,000-1:2,000

  • IP

  • 2-5 µg/ml.

  • IF-Cell

  • 1:200

  • FC

  • 1:1,000

靶点

功能

Myc gene encodes for a transcription factor that is believed to regulate expression of 15% of all genes through binding on Enhancer Box sequences (E-boxes) and recruiting histone acetyltransferases (HATs). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. This Myc-Tag antibody detects Myc-tagged fusion proteins.

背景文献

1. "A quantitative atlas of mitotic phosphorylation."Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008)

2. "Transactivation of gene expression by Myc is inhibited by mutation at the phosphorylation sites Thr-58 and Ser-62."Gupta S., Seth A., Davis R.J. Proc. Natl. Acad. Sci. U.S.A. 90:3216-3220(1993)

翻译后修饰

Phosphorylated by PRKDC. Phosphorylation at Ser-329 by PIM2 leads to the stabilization of MYC (By similarity). Phosphorylation at Ser-62 by CDK2 prevents Ras-induced senescence. Phosphorylated at Ser-62 by DYRK2; this primes the protein for subsequent phosphorylation by GSK3B at Thr-58. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.; Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28 but by USP36, due to the lack of interaction between isoform 3 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex. Ubiquitinated by TRIM6 in a phosphorylation-independent manner (By similarity).

UNIPROT #

SwissProt: P01106 Human

别名

avian myelocytomatosis viral oncogene homolog antibody

bHLHe39 antibody

c-Myc antibody

class E basic helix-loop-helix protein 39 antibody

MRTL antibody

MYC antibody

Myc Epitope Tag antibody

myc proto-oncogene protein antibody

myc-related translation/localization regulatory factor antibody

oncogene c-Myc antibody

展开

图片

  • Western blot analysis of Myc tag on PG-CM lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/R1208-1" style="font-weight: bold;text-decoration: underline;">R1208-1</a>, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Myc tag on PG-CM lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (R1208-1, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Myc tag on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/R1208-1" style="font-weight: bold;text-decoration: underline;">R1208-1</a>, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.<br />Positive control: <br />Lane 1: C-terminal Myc-tagged recombinant protein<br />Lane 2: N-terminal Myc-tagged recombinant protein

    Western blot analysis of Myc tag on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (R1208-1, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: C-terminal Myc-tagged recombinant protein
    Lane 2: N-terminal Myc-tagged recombinant protein

  • Myc tag was immunoprecipitated in 2µg C terminal Myc Tag fusion protein lysate with <a href="/products/R1208-1" style="font-weight: bold;text-decoration: underline;">R1208-1</a> at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using <a href="/products/EM31105" style="font-weight: bold;text-decoration: underline;">EM31105</a> at 1/1000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:20,000 dilution was used for 60 mins at room temperature.<br /><br />Lane 1: Myc Tag fusion protein lysate (input).<br />Lane 2: <a href="/products/R1208-1" style="font-weight: bold;text-decoration: underline;">R1208-1</a> IP in Myc Tag fusion protein lysate.<br />Lane 3: Rabbit IgG instead of <a href="/products/R1208-1" style="font-weight: bold;text-decoration: underline;">R1208-1</a> in Myc Tag fusion protein lysate.<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST

    Myc tag was immunoprecipitated in 2µg C terminal Myc Tag fusion protein lysate with R1208-1 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using EM31105 at 1/1000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 60 mins at room temperature.

    Lane 1: Myc Tag fusion protein lysate (input).
    Lane 2: R1208-1 IP in Myc Tag fusion protein lysate.
    Lane 3: Rabbit IgG instead of R1208-1 in Myc Tag fusion protein lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST

  • Myc tag was immunoprecipitated in 2µg N terminal Myc Tag fusion protein lysate with <a href="/products/R1208-1" style="font-weight: bold;text-decoration: underline;">R1208-1</a> at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using <a href="/products/EM31105" style="font-weight: bold;text-decoration: underline;">EM31105</a> at 1/1000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:20,000 dilution was used for 60 mins at room temperature.<br /><br />Lane 1: Myc Tag fusion protein lysate (input).<br />Lane 2: <a href="/products/R1208-1" style="font-weight: bold;text-decoration: underline;">R1208-1</a> IP in Myc Tag fusion protein lysate.<br />Lane 3: Rabbit IgG instead of <a href="/products/R1208-1" style="font-weight: bold;text-decoration: underline;">R1208-1</a> in Myc Tag fusion protein lysate.<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST

    Myc tag was immunoprecipitated in 2µg N terminal Myc Tag fusion protein lysate with R1208-1 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using EM31105 at 1/1000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 60 mins at room temperature.

    Lane 1: Myc Tag fusion protein lysate (input).
    Lane 2: R1208-1 IP in Myc Tag fusion protein lysate.
    Lane 3: Rabbit IgG instead of R1208-1 in Myc Tag fusion protein lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST

  • Immunocytochemistry analysis of 293T cells labeling Myc tag with Rabbit anti-Myc tag antibody (<a href="/products/R1208-1" style="font-weight: bold;text-decoration: underline;">R1208-1</a>) at 1/200 dilution.<br /><br />293T cells, transfected with Myc-tagged empty control, Claudin18.2 (C-terminal) or Histone H3.1 (N-terminal) expression vector, respectively, were fixed in 4% paraformaldehyde for 10 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Myc tag antibody (<a href="/products/R1208-1" style="font-weight: bold;text-decoration: underline;">R1208-1</a>) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 594, <a href="/products/HA1122" style="font-weight: bold;text-decoration: underline;">HA1122</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Myc Tag (<a href="/products/HA601081" style="font-weight: bold;text-decoration: underline;">HA601081</a>, green) was stained at 1/1,000 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of 293T cells labeling Myc tag with Rabbit anti-Myc tag antibody (R1208-1) at 1/200 dilution.

    293T cells, transfected with Myc-tagged empty control, Claudin18.2 (C-terminal) or Histone H3.1 (N-terminal) expression vector, respectively, were fixed in 4% paraformaldehyde for 10 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Myc tag antibody (R1208-1) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Myc Tag (HA601081, green) was stained at 1/1,000 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution.

  • Flow cytometric analysis of 293T cells transfected C-myc-tag labeling Myc tag.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/R1208-1" style="font-weight: bold;text-decoration: underline;">R1208-1</a>, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of 293T cells transfected C-myc-tag labeling Myc tag.

    Cells were fixed and permeabilized. Then stained with the primary antibody (R1208-1, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. ICC
  2. WB
  3. CO-IP
  4. IP
  5. Co-IP
  6. CoIP
  7. IF
  8. IB

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metafield image

HRP Conjugated Myc tag Rabbit Polyclonal Antibody

Application: WB

Reactivity: Species independent

Conjugate: HRP