MHC Class II Recombinant Rabbit Monoclonal Antibody [JA10-94] - BSA and Azide free
Catalog# HA750404
MHC Class II Recombinant Rabbit Monoclonal Antibody [JA10-94] - BSA and Azide free
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WB
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IP
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IF-Cell
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IF-Tissue
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IHC-P
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Human
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ET1704-13
含抗保成分
-
unconjugated
概述
产品名称
MHC Class II Recombinant Rabbit Monoclonal Antibody [JA10-94] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human HLA-DPB1 aa 11-60 / 258.
种属反应性
Human
验证应用
WB, IP, IF-Cell, IF-Tissue, IHC-P
分子量
Predicted band size: 29 kDa
阳性对照
Daudi cell lysates, human tonsil tissue, human endometrium tissue, Daudi.
偶联
unconjugated
克隆号
JA10-94
产品特性
形态
Liquid
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
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1:5,000
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IF-Cell
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1:50
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IF-Tissue
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1:50-1:100
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IHC-P
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1:1,000
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IP
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Use at an assay dependent concentration.
靶点
功能
Major histocompatibility complex (MHC) molecules, also designated human leukocyte antigen (HLA) molecules, are cell-surface receptors that bind foreign peptides and present them to T lymphocytes. MHC class I molecules consist of two polypeptide chains, an α or heavy chain and β-2-Microglobulin, a non-covalently associated protein. Cytotoxic T lymphocytes bind antigenic peptides presented by MHC class I molecules. Antigens that bind to MHC class I molecules are typically eight to ten residues in length and are stabilized in a peptide binding groove. MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an α and β chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumu-lating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.
背景文献
1. Feng M et al. ALV-J strain SCAU-HN06 induces innate immune responses in chicken primary monocyte-derived macrophages. Poult Sci N/A:N/A (2016).
2. Arebro J et al. Antigen-presenting epithelial cells can play a pivotal role in airway allergy. J Allergy Clin Immunol N/A:N/A (2015).
序列相似性
Belongs to the MHC class II family.
亚细胞定位
Cell membrane. Endoplasmic reticulum membrane.
UNIPROT
别名
D6S221E antibody
DMA antibody
DMB antibody
DP beta 1 antibody
DP beta 1 chain antibody
DP(W4) beta chain antibody
DPB 1 antibody
DPB1 antibody
DPB1_HUMAN antibody
DRB antibody
展开图片
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Western blot analysis of MHC Class II on Daudi cell lysates with Rabbit anti-MHC Class II antibody (HA750404) at 1/500 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 1 minute;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750404) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-MHC Class II antibody (HA750404) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750404) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human endometrium tissue with Rabbit anti-MHC Class II antibody (HA750404) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750404) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunocytochemistry analysis of Daudi cells labeling MHC Class II with Rabbit anti-MHC Class II antibody (HA750404) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MHC Class II antibody (HA750404) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
MHC Class II Recombinant Rabbit Monoclonal Antibody [JA10-94]
Application: WB,IP,IF-Cell,IF-Tissue,IHC-P,mIHC
Reactivity: Human
Conjugate: unconjugated
