PTP1B Mouse Monoclonal Antibody [D0-C7]
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Catalog# M1511-7
PTP1B Mouse Monoclonal Antibody [D0-C7]
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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Rat
概述
产品名称
PTP1B Mouse Monoclonal Antibody [D0-C7]
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein within human PTP1B aa 1-250.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Cell, IHC-P
分子量
Predicted band size: 50 kDa
阳性对照
A431 cell lysate, Hela cell lysate, A549 cell lysate, HepG2 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, C6 cell lysate, A431, human lung cancer tissue, mouse lung tissue.
偶联
unconjugated
克隆号
D0-C7
RRID
产品特性
形态
Liquid
浓度
2ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000-1:2,000
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IF-Cell
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1:100-1:500
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IHC-P
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1:200-1:1,000
发表文章中的应用
| WB | 查看 1 篇文献如下 |
发表文章中的种属
| CMECs | 查看 1 篇文献如下 |
靶点
功能
The phosphorylation of proteins at tyrosine residues has long been recognized as an important regulatory component of signal transduction. This is a reversible process, involving both enzymes that phosphorylate proteins on tyrosine residues as well as a rapidly expanding family of protein tyrosine phosphatases. These latter enzymes bear little resemblance to either the protein serine and protein threonine phosphatases or to the acid and alkaline phosphatases. In most tissues, the major PTPase is a vanadate- and molybdate-sensitive protein. On the basis of sequence analysis, PTP1B (PTPase 1B) expressed in human placenta exhibits similarities both with the common leukocyte antigen (CD45) and with LAR, a homolog of the neural adhesion molecule (NCAM). PTP1B is synthesized as a 435 amino acid precursor protein which is cleaved to generate the active 321 amino acid enzyme.
背景文献
1. Borges Bde C, et al. Protein tyrosine phosphatase-1B contributes to LPS-induced leptin resistance in male rats. Am J Physiol Endocrinol Metab 308:E40-50 (2015).
2. Krishnan N., et al. H2s-induced sulfhydration of the phosphatase PTP1B and its role in the endoplasmic reticulum stress response. Sci. Signal. 4:RA86-RA86(2011).
序列相似性
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
组织特异性
Expressed in keratinocytes (at protein level).
翻译后修饰
Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a sulfenamide cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.; Ser-50 is the major site of phosphorylation as compared to Ser-242 and Ser-243. Activated by phosphorylation at Ser-50.; S-nitrosylation of Cys-215 inactivates the enzyme activity.; Sulfhydration at Cys-215 following endoplasmic reticulum stress inactivates the enzyme activity, promoting EIF2AK3/PERK activity.
亚细胞定位
Endoplasmic reticulum membrane
别名
PTP1B antibody
Non receptor tyrosine phosphatase 1 antibody
Protein phosphotyrosylphosphatase 1B antibody
Protein tyrosine phosphatase 1B antibody
Protein tyrosine phosphatase non receptor type 1 antibody
Protein tyrosine phosphatase placental antibody
Protein-tyrosine phosphatase 1B antibody
PTN1_HUMAN antibody
PTP 1B antibody
PTP-1B antibody
展开图片
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☑ Knockdown (KD)
Western blot analysis of PTP1B on different lysates with Mouse anti-PTP1B antibody (M1511-7) at 1/1,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-PTP1B KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1511-7) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of PTP1B on different lysates with Mouse anti-PTP1B antibody (M1511-7) at 1/1,000 dilution.
Lane 1: RAW264.7 cell lysate
Lane 2: PC-12 cell lysate
Lane 3: C6 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 43 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1511-7) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of PTP1B on different lysates with Mouse anti-PTP1B antibody (M1511-7) at 1/500 dilution.
Lane 1: A431 cell lysate
Lane 2: Hela cell lysate
Lane 3: A549 cell lysate
Lane 4: HepG2 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1511-7) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of A431 cells labeling PTP1B with Mouse anti-PTP1B antibody (M1511-7) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PTP1B antibody (M1511-7) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Mouse anti-PTP1B antibody (M1511-7) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1511-7) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Mouse anti-PTP1B antibody (M1511-7) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1511-7) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Angiotensin II induces apoptosis of cardiac microvascular endothelial cells via regulating PTP1B/PI3K/Akt pathway.
Author: Jingmin Zhou,Junbo Ge
PMID: 31502193
应用: WB
反应种属: CMECs
发表时间: 2019 Dec
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Citation
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Conjugate: unconjugated
