VSV-G-tag Mouse Monoclonal Antibody [E0-G7]
Catalog# M1510-10
VSV-G-tag Mouse Monoclonal Antibody [E0-G7]
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WB
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IP
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ELISA
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Species independent
概述
产品名称
VSV-G-tag Mouse Monoclonal Antibody [E0-G7]
抗体类型
Mouse Monoclonal Antibody
免疫原
Synthetic peptide.
种属反应性
Species independent
验证应用
WB, IP, ELISA
阳性对照
Recombinant VSV-G-tag protein.
偶联
unconjugated
克隆号
E0-G7
RRID
产品特性
形态
Liquid
浓度
2ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
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1:1,000-1:20,000
靶点
功能
Vesicular Stomatitis Virus (VSV) is an arbovirus in the family Rhabdoviridae, order Mononegavirales. VSV infects insects and mammals. The genome of VSV is a negative-sense RNA strand that encodes five major proteins: glycoprotein (G), matrix protein (M), nucleoprotein (NC), large protein (L) and phosphoprotein. The L protein and the phosphoprotein combine to catalyze the replication of VSV’s mRNA. After endocytosis, the G protein facilitates VSV’s entry into the cell by controlling virus attachment to the host cell as well as fusion of the viral envelope with the endosomal membrane. Transport of the G protein from the endoplasmic reticulum (ER) to the plasma membrane (PM) is temperature sensitive. Because of this property, VSV is commonly used in research labs to study the properties of viruses in the Rhabdoviridae family, and to study viral evolution.
背景文献
1. Niemel EH et al. Global analysis of the nuclear processing of transcripts with unspliced U12-type introns by the exosome. Nucleic Acids Res 42:7358-69 (2014).
2. J ger W et al. Ultrasound-guided intramural inoculation of orthotopic bladder cancer xenografts: a novel high-precision approach. PLoS One 8:e59536 (2013).
别名
Vesicular stomatitis virus glycoprotein tag antibody
VSV epitope tag antibody
VSV tag antibody
VSV-G epitope tag antibody
VSVG antibody
vsvg tag antibody
图片
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Western blot analysis of VSV-G-tag on recombinant VSV-G-tag protein with Mouse anti-VSV-G-tag antibody (M1510-10) at different dilutions.
Lysates/proteins at 50 ng/Lane.
Exposure time: 15 seconds;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1510-10) at different dilutions was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"