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ALDH2 Mouse Monoclonal Antibody [E4-D10]

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Catalog# M1509-1

ALDH2 Mouse Monoclonal Antibody [E4-D10]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

概述

产品名称

ALDH2 Mouse Monoclonal Antibody [E4-D10]

抗体类型

Mouse Monoclonal Antibody

免疫原

Synthetic peptide within Human ALDH2 aa 468-517 / 517.

种属反应性

Human, Mouse, Rat

验证应用

WB, IF-Cell, IF-Tissue, IHC-P, FC

分子量

Predicted band size: 56 kDa

阳性对照

HepG2 cell lysate, A549 cell lysate, NIH/3T3 cell lysate, SK-Br-3, HeLa, human lung cancer tissue, human liver cancer tissue, human colon cancer tissue, human gastric cancer tissue, mouse colon tissue, mouse stomach tissue.

偶联

unconjugated

克隆号

E4-D10

RRID

AB_3073118

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG1

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:1,000-1:5,000

  • IF-Cell

  • 1:100

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

靶点

功能

Aldehyde dehydrogenases (ALDHs) mediate NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde; lipid peroxidation; and metabolism of corticosteroids, biogenic amines and neurotransmitters. ALDH1A1, also designated retinal dehydrogenase 1 (RalDH1 or RALDH1); aldehyde dehydrogenase family 1 member A1; aldehyde dehydrogenase cytosolic; ALDHII; ALDH-E1 or ALDH E1, is a retinal dehydrogenase that participates in the biosynthesis of retinoic acid (RA). The major liver isoform ALDH1 localizes to cytosolic space, while ALDH2 localizzes to the mitochondria. The ALDH1A2 (RALDH2, RALDH2-T) gene produces three different transcripts and also catalyzes the synthesis of RA from retinaldehyde. ALDH2 is present in most Caucasians, yet is absent in 50% of Asians. The absence of this enzyme has been linked to alcohol intolerance; and thusly, a reduced risk for alcoholism-related liver disease.

背景文献

1. Yu YH et al. PKC-ALDH2 Pathway Plays a Novel Role in Adipocyte Differentiation. PLoS One 11:e0161993 (2016).

2. Ferrand N et al. Loss of WISP2/CCN5 in estrogen-dependent MCF7 human breast cancer cells promotes a stem-like cell phenotype. PLoS One 9:e87878 (2014).

序列相似性

Belongs to the aldehyde dehydrogenase family.

亚细胞定位

Mitochondrion matrix.

UNIPROT

SwissProt: P05091 Human

SwissProt: P47738 Mouse

SwissProt: P11884 Rat

别名

Acetaldehyde dehydrogenase 2 antibody

Aldehyde dehydrogenase 2 family (mitochondrial) antibody

Aldehyde dehydrogenase 2 family antibody

Aldehyde dehydrogenase mitochondrial antibody

Aldehyde dehydrogenase, mitochondrial antibody

ALDH 2 antibody

ALDH class 2 antibody

ALDH E2 antibody

ALDH-E2 antibody

Aldh2 antibody

展开

图片

  • Western blot analysis of ALDH2 on different lysates with Mouse anti-ALDH2 antibody (<a href="/products/M1509-1" style="font-weight: bold;text-decoration: underline;">M1509-1</a>) at 1/1,000 dilution.<br /><br />Lane 1: HepG2 cell lysate (20 µg/Lane)<br />Lane 2: A549 cell lysate (20 µg/Lane)<br />Lane 3: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 4: Mouse liver tissue lysate (40 µg/Lane)<br />Lane 5: Rat liver tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 56 kDa<br />Observed band size: 50 kDa<br /><br />Exposure time: 40 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/M1509-1" style="font-weight: bold;text-decoration: underline;">M1509-1</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ALDH2 on different lysates with Mouse anti-ALDH2 antibody (M1509-1) at 1/1,000 dilution.

    Lane 1: HepG2 cell lysate (20 µg/Lane)
    Lane 2: A549 cell lysate (20 µg/Lane)
    Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 4: Mouse liver tissue lysate (40 µg/Lane)
    Lane 5: Rat liver tissue lysate (40 µg/Lane)

    Predicted band size: 56 kDa
    Observed band size: 50 kDa

    Exposure time: 40 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1509-1) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of SK-Br-3 cells labeling ALDH2 with Mouse anti-ALDH2 antibody (<a href="/products/M1509-1" style="font-weight: bold;text-decoration: underline;">M1509-1</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-ALDH2 antibody (<a href="/products/M1509-1" style="font-weight: bold;text-decoration: underline;">M1509-1</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />beta Tubulin (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 594, <a href="/products/HA1122" style="font-weight: bold;text-decoration: underline;">HA1122</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of SK-Br-3 cells labeling ALDH2 with Mouse anti-ALDH2 antibody (M1509-1) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-ALDH2 antibody (M1509-1) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.

  • Immunocytochemistry analysis of HeLa cells labeling ALDH2 with Mouse anti-ALDH2 antibody (<a href="/products/M1509-1" style="font-weight: bold;text-decoration: underline;">M1509-1</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-ALDH2 antibody (<a href="/products/M1509-1" style="font-weight: bold;text-decoration: underline;">M1509-1</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />beta Tubulin (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 594, <a href="/products/HA1122" style="font-weight: bold;text-decoration: underline;">HA1122</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HeLa cells labeling ALDH2 with Mouse anti-ALDH2 antibody (M1509-1) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-ALDH2 antibody (M1509-1) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.

  • Immunohistochemical analysis of paraffin- embedded human lung cancer tissue using anti-ALDH2 Mouse mAb.

    Immunohistochemical analysis of paraffin- embedded human lung cancer tissue using anti-ALDH2 Mouse mAb.

  • Immunohistochemical analysis of paraffin- embedded human liver cancer tissue using anti-ALDH2 Mouse mAb.

    Immunohistochemical analysis of paraffin- embedded human liver cancer tissue using anti-ALDH2 Mouse mAb.

  • Immunohistochemical analysis of paraffin- embedded human colon cancer tissue using anti-ALDH2 Mouse mAb.

    Immunohistochemical analysis of paraffin- embedded human colon cancer tissue using anti-ALDH2 Mouse mAb.

  • Immunohistochemical analysis of paraffin- embedded human gastric cancer tissue using anti-ALDH2 Mouse mAb.

    Immunohistochemical analysis of paraffin- embedded human gastric cancer tissue using anti-ALDH2 Mouse mAb.

  • Immunohistochemical analysis of paraffin- embedded mouse colon tissue using anti-ALDH2 Mouse mAb.

    Immunohistochemical analysis of paraffin- embedded mouse colon tissue using anti-ALDH2 Mouse mAb.

  • Immunohistochemical analysis of paraffin- embedded mouse stomach tissue using anti-ALDH2 Mouse mAb.

    Immunohistochemical analysis of paraffin- embedded mouse stomach tissue using anti-ALDH2 Mouse mAb.

  • Flow cytometric analysis of HepG2 cells with ALDH2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary antibody.

    Flow cytometric analysis of HepG2 cells with ALDH2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary antibody.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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