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6X His tag Mouse Monoclonal Antibody [8-F0]

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Catalog# M0812-3

6X His tag Mouse Monoclonal Antibody [8-F0]

Application

  • WB

  • IP

Reactivity

  • Species independent

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

6X His tag Mouse Monoclonal Antibody [8-F0]

抗体类型

Mouse Monoclonal Antibody

免疫原

Synthetic peptide immune sequence is CHHHHHHHH.

种属反应性

Species independent

验证应用

WB, IP

阳性对照

C-terminal His-tagged recombinant protein.

偶联

unconjugated

克隆号

8-F0

RRID

AB_3072999

产品特性

形态

Liquid

浓度

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol.

亚型

IgG2a

纯化方式

Protein G affinity purified.

应用稀释度

  • WB

  • 1:2,000

  • IP

  • 2-5 µg/ml.

靶点

功能

A variety of plasmids contain DNA that encodes a tag consisting of six histidine (6xHis) residues followed by an extended multiple cloning site. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography. This antibody can be used to detect the carboxyl-terminal 6×his tag in recombinant protein.

背景文献

暂无

别名

6 His epitope tag antibody

Hexa His tag antibody

HHHHHH epitope tag antibody

HHHHHH tag antibody

His tag antibody

Polyhistidine Tag antibody

图片

  • His tag was immunoprecipitated in 2µg C terminal His Tag fusion protein lysate with <a href="/products/M0812-3" style="font-weight: bold;text-decoration: underline;">M0812-3</a> at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using <a href="/products/R1207-2" style="font-weight: bold;text-decoration: underline;">R1207-2</a> at 1/500 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 60 mins at room temperature.<br /><br />Lane 1: His Tag fusion protein lysate (input).<br />Lane 2: <a href="/products/M0812-3" style="font-weight: bold;text-decoration: underline;">M0812-3</a> IP in His Tag fusion protein lysate.<br />Lane 3: Mouse IgG instead of <a href="/products/M0812-3" style="font-weight: bold;text-decoration: underline;">M0812-3</a> in His Tag fusion protein lysate.<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST

    His tag was immunoprecipitated in 2µg C terminal His Tag fusion protein lysate with M0812-3 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using R1207-2 at 1/500 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 60 mins at room temperature.

    Lane 1: His Tag fusion protein lysate (input).
    Lane 2: M0812-3 IP in His Tag fusion protein lysate.
    Lane 3: Mouse IgG instead of M0812-3 in His Tag fusion protein lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST

  • Western blot analysis of 6X His tag on His-tagged recombinant protein with Mouse anti-6X His tag antibody (<a href="/products/M0812-3" style="font-weight: bold;text-decoration: underline;">M0812-3</a>) at 1/5,000 dilution.<br /><br />Lane 1: N-terminal His-tagged recombinant protein<br />Lane 2: C-terminal His-tagged recombinant protein<br /><br />Lysates/proteins at 50 ng/Lane.<br />Exposure time: 100 seconds; ECL: K1802<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/M0812-3" style="font-weight: bold;text-decoration: underline;">M0812-3</a>, 1/5,000 in 5% NFDM/TBST, overnight at 4 ℃<br />Secondary antibody: Goat anti-Mouse IgG-HRP (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 30 kDa<br />Observed band size: 30 kDa

    Western blot analysis of 6X His tag on His-tagged recombinant protein with Mouse anti-6X His tag antibody (M0812-3) at 1/5,000 dilution.

    Lane 1: N-terminal His-tagged recombinant protein
    Lane 2: C-terminal His-tagged recombinant protein

    Lysates/proteins at 50 ng/Lane.
    Exposure time: 100 seconds; ECL: K1802

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: M0812-3, 1/5,000 in 5% NFDM/TBST, overnight at 4 ℃
    Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 30 kDa
    Observed band size: 30 kDa

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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