CD34 Recombinant Rabbit Monoclonal Antibody
Catalog# IRS050
CD34 Recombinant Rabbit Monoclonal Antibody
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mIHC
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Mouse
概述
产品名称
CD34 Recombinant Rabbit Monoclonal Antibody
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human CD34 aa 336-385 / 385.
种属反应性
Mouse
验证应用
mIHC
分子量
Predicted band size: 41 kDa
阳性对照
Mouse liver tissue, mouse brain tissue, mouse spleen tissue.
偶联
unconjugated
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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mIHC
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1:100
靶点
功能
CD34 is a heavily glycosylated, transmembrane glycoprotein that is expressed on the surface of lymphohematopoietic stem and progenitor cells, small-vessel endothelial cells, embryonic fibroblasts and some cells in fetal and adult nervous tissue. CD34 antigen expression is highest in the most primitive stem cells and is gradually lost as lineage committed progenitors differentiate. The CD34 antigen is also present on capillary endothelial cells and on bone marrow stromal cells. The CD34 cytoplasmic domain has an intracellular domain that contains consensus sites for activated protein kinase C (PKC) phosphorylation as well as serine, threonine and tyrosine phosphorylation consensus sites.
背景文献
1. Lin, SZ. et al. 2015. Emodin inhibits angiogenesis in pancreatic cancer by regulating the transforming growth factor-β/drosophila mothers against decapentaplegic pathway and angiogenesis-associated microRNAs. Molecular medicine reports. 12: 5865-71.
2. Corradi, LS. et al. 2013. Structural and ultrastructural evidence for telocytes in prostate stroma. J. Cell. Mol. Med. 17: 398-406.
序列相似性
Belongs to the CD34 family.
组织特异性
Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
翻译后修饰
Highly glycosylated.; Phosphorylated on serine residues by PKC.
亚细胞定位
Membrane.
UNIPROT
别名
CD34 antibody
CD34 antigen antibody
CD34 molecule antibody
CD34_HUMAN antibody
Cluster designation 34 antibody
Hematopoietic progenitor cell antigen CD34 antibody
HPCA1 antibody
Mucosialin antibody
OTTHUMP00000034733 antibody
OTTHUMP00000034734 antibody
图片
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mIHC analysis of mouse liver tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD34 antibody (IRS050) at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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mIHC analysis of mouse brain tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD34 antibody (IRS050) at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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mIHC analysis of mouse spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD34 antibody (IRS050) at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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mIHC analysis of mouse brain tissue (Formalin/PFA-fixed paraffin-embedded sections) with SOX2, TBR1 (IRS070), Olig2 (IRS067), CD34 (IRS050) and GFAP (IRS069) antibody at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"