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IL-1 beta Recombinant Rabbit Monoclonal Antibody [JJ087-3]

RMB: 900 特惠 1500

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Catalog# ET1701-39

IL-1 beta Recombinant Rabbit Monoclonal Antibody [JJ087-3]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

Reactivity

  • Human

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

IL-1 beta Recombinant Rabbit Monoclonal Antibody [JJ087-3]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within C-terminal human IL1 beta.

种属反应性

Human

验证应用

WB, IF-Cell, IF-Tissue, IHC-P

分子量

Predicted band size: 31 kDa

阳性对照

THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours and 300ng/mL BFA for 3 hours whole cell lysate, THP-1, human kidney tissue.

偶联

unconjugated

克隆号

JJ087-3

RRID

AB_3070207

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:5,000

  • IF-Cell

  • 1:50-1:200

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

靶点

功能

Two forms of interleukin-1, designated IL-1α and IL-1β, have been described. Although encoded by distinct genes and exhibiting roughly only 25% sequence identity, IL-1α and IL-1β bind to the same receptor and seem to elicit similar biological responses. IL-1 production is generally thought to be associated with inflammation, but it has also been shown to be expressed during kidney development, thymocyte differentiation and cartilage degradation. IL-1 plays a critical role in the regulation of immune response and inflammation, acting as an activator of T and B lymphocytes and natural killer (NK) cells. In T cells, IL-1 stimulates the production of IL-2 and selectively inhibits IL-4 expression. IL-1 induces B cell proliferation and maturation, and immunoglobulin synthesis. NK cells require IL-1β for production of the anti- pathogen IFN-γ. IL-1 has also been implicated in several pathological conditions including rheumatoid arthritis, inflammatory bowel disease and atherosclerosis.

背景文献

1. Ashok A et al. Exposure to As-, Cd-, and Pb-mixture induces A , amyloidogenic APP processing and cognitive impairments via oxidative stress-dependent neuroinflammation in young rats. Toxicol Sci 143:64-80 (2015).

2. Zhang J et al. Effects of p75 neurotrophin receptor on regulating hypoxia-induced angiogenic factors in retinal pigment epithelial cells. Mol Cell Biochem 398:123-34 (2015).

序列相似性

Belongs to the IL-1 family.

组织特异性

Expressed in activated monocytes/macrophages (at protein level).

翻译后修饰

Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.

亚细胞定位

Cytoplasm, Lysosome, extracellular exosome, Secreted.

UNIPROT

SwissProt: P01584 Human

别名

Catabolin antibody

H1 antibody

IL 1 antibody

IL 1 beta antibody

IL-1 beta antibody

IL1 BETA antibody

IL1B antibody

IL1B_HUMAN antibody

IL1F2 antibody

Interleukin 1 beta antibody

展开

图片

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of IL-1 beta on different lysates with Rabbit anti-IL-1 beta antibody (<a href="/products/ET1701-39" style="font-weight: bold;text-decoration: underline;">ET1701-39</a>) at 1/5,000 dilution.<br /><br />Lane 1: THP-1 whole cell lysate<br />Lane 2: THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours and 300ng/mL BFA for 3 hours whole cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 31 kDa<br />Observed band size: 34 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1701-39" style="font-weight: bold;text-decoration: underline;">ET1701-39</a>) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of IL-1 beta on different lysates with Rabbit anti-IL-1 beta antibody (ET1701-39) at 1/5,000 dilution.

    Lane 1: THP-1 whole cell lysate
    Lane 2: THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours and 300ng/mL BFA for 3 hours whole cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 31 kDa
    Observed band size: 34 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-39) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Immunocytochemistry analysis of THP-1 cells untreated with or without TPA(80nM overnight) then LPS(100ng/mL 6h)+BFA(300ng/mL 3h) labeling IL-1 beta with Rabbit anti-IL-1 beta antibody (<a href="/products/ET1701-39" style="font-weight: bold;text-decoration: underline;">ET1701-39</a>) at 1/500 dilution.<br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IL-1 beta antibody (<a href="/products/ET1701-39" style="font-weight: bold;text-decoration: underline;">ET1701-39</a>) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    ☑ Cell treatment (CT)

    Immunocytochemistry analysis of THP-1 cells untreated with or without TPA(80nM overnight) then LPS(100ng/mL 6h)+BFA(300ng/mL 3h) labeling IL-1 beta with Rabbit anti-IL-1 beta antibody (ET1701-39) at 1/500 dilution.
    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IL-1 beta antibody (ET1701-39) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-IL-1 beta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1701-39" style="font-weight: bold;text-decoration: underline;">ET1701-39</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-IL-1 beta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-39, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB
  2. IHC-P
  3. mIHC
  4. IF

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