Biotin Conjugated Human VEGF Receptor 1 Recombinant Rabbit Monoclonal Antibody [PSH19-23] - BSA and Azide free (Detector)

Sandwich ELISA analysis of Human VEGF Receptor 1 matched pair antibodies

Capture: HA725365, Human VEGF Receptor 1 Rabbit mAb [PSH19-22]
Detector: HA725366, Human VEGF Receptor 1 Rabbit mAb [PSH19-23]

Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725365) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human VEGF Receptor 1 protein (HA211083) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725366, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native VEGFR1(FLT1) in human plasma samples.

Capture: HA725365, Human VEGF Receptor 1 Rabbit mAb [PSH19-22]
Detector: HA725366, Human VEGF Receptor 1 Rabbit mAb [PSH19-23]

The concentrations of VEGFR1(FLT1) were measured in duplicates, interpolated from the VEGFR1(FLT1) standard curve and corrected for sample dilution. Undiluted samples are human plasma 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGFR1(FLT1) concentration was determined to be 712.28 pg/ml in human plasma.