Human SAA1 DTSet enzyme-linked immunoassay kit
概述
产品名称
Human SAA1 DTSet enzyme-linked immunoassay kit
产品类型
DTSet Elisa Kit
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0215 | 0.0202 | 0.0209 | |
| 312.50 | 0.0341 | 0.0357 | 0.0349 | 0.0141 |
| 625.00 | 0.0522 | 0.0509 | 0.0516 | 0.0307 |
| 1250.00 | 0.0863 | 0.0880 | 0.0872 | 0.0663 |
| 2500.00 | 0.1977 | 0.1817 | 0.1897 | 0.1689 |
| 5000.00 | 0.5393 | 0.5431 | 0.5412 | 0.5204 |
| 10000.00 | 1.5540 | 1.4910 | 1.5225 | 1.5017 |
| 20000.00 | 3.3746 | 3.4427 | 3.4087 | 3.3878 |
实验类型
夹心酶联免疫吸附测定(Sandwich Elisa)
种属反应性
Human
检测方法
比色法
实验检测时长
120min
标准曲线范围
312.5 pg/ml -20000 pg/ml
标准曲线梯度
7点/3倍
孵育次数
2
可检测样本
加样体积
50 μl
产品特性
- 优化的捕获和检测抗体配对与推荐浓度可节省较长的开发时间
- 提供开发方案以指导进一步的分析优化
- 检测可根据您的特定需求进行定制
- 完整试剂盒的经济替代品
试剂盒组分
- 捕获抗体
- 检测抗体
- 重组标准品
- 与辣根过氧化物酶偶联的链霉亲和素 (Streptavidin-HRP)
所需的其他材料
DTSet辅助试剂盒(5板):包含96孔微孔板、封板膜、显色液、终止溶液、包板缓冲液(PBS)、洗涤缓冲液和检测缓冲液。
上面列出的部件可以单独购买:
- 96孔微孔板板(EP): YOUKE Life, Catalog # DSEP01
- 封板木膜(PS): YOUKE Life, Catalog # DSSF01。
- 包板缓冲液(CB): 137mM NaCl,2.7mM KCl,8.1mM Na2HPO4,1.5mM KH2PO4,pH 7.2-7.4,0.2m过滤。YOUKE Life, Catalog # DSCB01。
- 检测缓冲液 10X(AB): 0.5%BSA,0.05%Tween20,PBS溶液。YOUKE Life, Catalog # DSAB01。
- 封闭液(BB): YOUKE Life, Catalog # DSBB01。
- Washing Solution: 0.05%Tween20,PBS溶液,pH 7.2-7.4。YOUKE Life, Catalog # DSWB01。
- 底物显色液(TMB): TMB。YOUKE Life, Catalog # DSTS01。
- 终止溶液(SS): 0.5mol/ml H2SO4。YOUKE Life, Catalog # DSSS01。
靶点
功能
小鼠血清淀粉样蛋白A-1(SAA1)是一种多功能载脂蛋白,由肝细胞响应促炎细胞因子产生。它以12 kDa、103氨基酸(AA)组成的非糖基化多肽形式分泌,作为HDL复合物的一部分循环。SAA1基因是小鼠中三种SAA基因之一,基于人类,很可能具有等位基因。SAA1基因产物与SAA2基因产物仅有九个氨基酸的区别。在人类中,循环中的SAA1显示出多种蛋白水解产生的异构体,其中N端或C端可切割出一到三种氨基酸。鼠标中也可能存在类似情况。成熟小鼠SAA1的氨基酸串行与成熟人类、兔子、马和仓鼠SAA1分别相同72%、72%、67%和74%。
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