Human IL-17E/IL-25 DTSet enzyme-linked immunoassay kit
概述
产品名称
Human IL-17E/IL-25 DTSet enzyme-linked immunoassay kit
产品类型
DTSet Elisa Kit
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0192 | 0.0210 | 0.0201 | |
| 62.50 | 0.1571 | 0.1715 | 0.1643 | 0.1442 |
| 125.00 | 0.3081 | 0.3098 | 0.3090 | 0.2889 |
| 250.00 | 0.6030 | 0.6163 | 0.6097 | 0.5896 |
| 500.00 | 1.2450 | 1.2580 | 1.2515 | 1.2314 |
| 1000.00 | 2.2880 | 2.3030 | 2.2955 | 2.2754 |
| 2000.00 | 3.6630 | 3.5170 | 3.5900 | 3.5699 |
| 4000.00 | 4.4157 | 4.4606 | 4.4382 | 4.4181 |
实验类型
夹心酶联免疫吸附测定(Sandwich Elisa)
种属反应性
Human
检测方法
比色法
实验检测时长
120min
标准曲线范围
62.50pg/ml-4000pg/ml
标准曲线梯度
7点/3倍
孵育次数
2
可检测样本
加样体积
50 μl
产品特性
- 优化的捕获和检测抗体配对与推荐浓度可节省较长的开发时间
- 提供开发方案以指导进一步的分析优化
- 检测可根据您的特定需求进行定制
- 完整试剂盒的经济替代品
试剂盒组分
- 捕获抗体
- 检测抗体
- 重组标准品
- 与辣根过氧化物酶偶联的链霉亲和素 (Streptavidin-HRP)
所需的其他材料
DTSet辅助试剂盒(5板):包含96孔微孔板、封板膜、显色液、终止溶液、包板缓冲液(PBS)、洗涤缓冲液和检测缓冲液。
上面列出的部件可以单独购买:
- 96孔微孔板板(EP): YOUKE Life, Catalog # DSEP01
- 封板木膜(PS): YOUKE Life, Catalog # DSSF01。
- 包板缓冲液(CB): 137mM NaCl,2.7mM KCl,8.1mM Na2HPO4,1.5mM KH2PO4,pH 7.2-7.4,0.2m过滤。YOUKE Life, Catalog # DSCB01。
- 检测缓冲液 10X(AB): 0.5%BSA,0.05%Tween20,PBS溶液。YOUKE Life, Catalog # DSAB01。
- 封闭液(BB): YOUKE Life, Catalog # DSBB01。
- Washing Solution: 0.05%Tween20,PBS溶液,pH 7.2-7.4。YOUKE Life, Catalog # DSWB01。
- 底物显色液(TMB): TMB。YOUKE Life, Catalog # DSTS01。
- 终止溶液(SS): 0.5mol/ml H2SO4。YOUKE Life, Catalog # DSSS01。
靶点
功能
IL-17家族由至少六种促炎细胞因子组成,它们共享保守的半胱氨酸结结构,但在N末端发散。IL-17家族成员是以二聚体形式分泌的糖蛋白,可诱导局部细胞因子产生并将粒细胞募集到炎症部位。IL-17由IL-15和IL-23诱导,主要存在于活化的CD4 + T细胞中,不同于Th1或Th2细胞。IL-17F与IL-17最同源,但仅由活化的单核细胞中的IL-23诱导。IL-17B 结合 IL-17B 受体,但不结合 IL-17 受体;它与IL-17D最同源,IL-17D由静息CD4 + T细胞和CD19 + B细胞表达。IL-17E主要由Th2细胞产生,并将嗜酸性粒细胞募集到肺组织中。IL-17C的表达模式非常有限,但在成人前列腺和胎儿肾脏库中已被检测到。
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