HRP Conjugated Human FAP Recombinant Rabbit Monoclonal Antibody [PSH26-56] - Detector
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概述
产品名称
HRP Conjugated Human FAP Recombinant Rabbit Monoclonal Antibody [PSH26-56] - Detector
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human FAP aa 26-760 (HA211419).
种属反应性
Human
验证应用
ELISA(Det), ELISA
阳性对照
Recombinant Human FAP protein (HA211419).
偶联
HRP
克隆号
PSH26-56
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH26-55] to Human FAP (Capture) (HA725499) and Recombinant Human FAP protein (HA211419) as the standard. The reference range value is 156.3-20,000 pg/mL.
-
ELISA
-
Use at an assay dependent concentration.
靶点
功能
FAP (fibroblast activation protein) is a cell surface glycoprotein and serine protease that is expressed primarily in fetal mesenchymal tissues and epithelial cancer fibroblasts. In cancer, FAP functions to promote cellular proliferation. In embryonic development, FAP functions to remodel developing tissues. FAP acts as an integral membrane gelatinase composed of N-glycosylated proteolytically inactive subunits. FAP expression on chondrocyte membranes is upregulated by the combination of the cytokines IL-1 and OSM and has been shown to increase in osteoarthritic patients. This expression is co-localized with MMP-1and MMP-13 as well as CD44 (variants v3 and v7/8). Mice that lack all copies of the FAP gene have been found to be fertile and to have developmental defects or change in cancer susceptibility.
背景文献
1. Jia, B. et al. 2016. GPR30 Promotes Prostate Stromal Cell Activation via Suppression of ERα Expression and Its Downstream Signaling Pathway. Endocrinology. 157: 3023-35.
2. Knopf, JD. et al. 2015. The stromal cell-surface protease fibroblast activation protein-α localizes to lipid rafts and is recruited to invadopodia. Biochim. Biophys. Acta. 1853: 2515-25.
亚细胞定位
Cell membrane. Cell surface.
UNIPROT
别名
170 kDa melanoma membrane bound gelatinase antibody
170 kDa melanoma membrane-bound gelatinase antibody
DPPIV antibody
FAP antibody
FAPA antibody
Fibroblast activation protein alpha antibody
Integral membrane serine protease antibody
SEPR_HUMAN antibody
Seprase antibody
图片
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![Sandwich ELISA analysis of Human FAP matched pair antibodies<br /><br />Capture: <a href="/products/HA725499" style="font-weight: bold;text-decoration: underline;">HA725499</a>, Human FAP Rabbit mAb [PSH26-55]<br />Detector: <a href="/products/HA725500" style="font-weight: bold;text-decoration: underline;">HA725500</a>, Human FAP Rabbit mAb [PSH26-56]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA725499" style="font-weight: bold;text-decoration: underline;">HA725499</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 5 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human FAP protein (<a href="/products/HA211419" style="font-weight: bold;text-decoration: underline;">HA211419</a>) starting from 20,000 pg/mL to 0 pg/mL and detect antibody (<a href="/products/HA725500" style="font-weight: bold;text-decoration: underline;">HA725500</a>, HRP, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725500H_1.jpg?v=20260908104421)
Sandwich ELISA analysis of Human FAP matched pair antibodies
Capture: HA725499, Human FAP Rabbit mAb [PSH26-55]
Detector: HA725500, Human FAP Rabbit mAb [PSH26-56]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725499) diluted in carbonate/bicarbonate buffer, at a concentration of 5 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human FAP protein (HA211419) starting from 20,000 pg/mL to 0 pg/mL and detect antibody (HA725500, HRP, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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