FAP Recombinant Rabbit Monoclonal Antibody
Catalog# IRS289RB
FAP Recombinant Rabbit Monoclonal Antibody
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mIHC
-
Human
-
unconjugated
概述
产品名称
FAP Recombinant Rabbit Monoclonal Antibody
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
A synthesized peptide derived from human FAP1
种属反应性
Human
验证应用
mIHC
分子量
Predicted band size: 88 kDa
阳性对照
Human cervical cancer tissue.
偶联
unconjugated
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
mIHC
-
1:100
靶点
功能
FAP (fibroblast activation protein) is a cell surface glycoprotein and serine protease that is expressed primarily in fetal mesenchymal tissues and epithelial cancer fibroblasts. In cancer, FAP functions to promote cellular proliferation. In embryonic development, FAP functions to remodel developing tissues. FAP acts as an integral membrane gelatinase composed of N-glycosylated proteolytically inactive subunits. FAP expression on chondrocyte membranes is upregulated by the combination of the cytokines IL-1 and OSM and has been shown to increase in osteoarthritic patients. This expression is co-localized with MMP-1and MMP-13 as well as CD44 (variants v3 and v7/8). Mice that lack all copies of the FAP gene have been found to be fertile and to have developmental defects or change in cancer susceptibility.
背景文献
1. Jia, B. et al. 2016. GPR30 Promotes Prostate Stromal Cell Activation via Suppression of ERα Expression and Its Downstream Signaling Pathway. Endocrinology. 157: 3023-35.
2. Knopf, JD. et al. 2015. The stromal cell-surface protease fibroblast activation protein-α localizes to lipid rafts and is recruited to invadopodia. Biochim. Biophys. Acta. 1853: 2515-25.
序列相似性
Belongs to the peptidase S9B family.
组织特异性
Expressed in adipose tissue. Expressed in the dermal fibroblasts in the fetal skin. Expressed in the granulation tissue of healing wounds and on reactive stromal fibroblast in epithelial cancers. Expressed in activated fibroblast-like synoviocytes from inflamed synovial tissues. Expressed in activated hepatic stellate cells (HSC) and myofibroblasts from cirrhotic liver, but not detected in normal liver. Expressed in glioma cells (at protein level). Expressed in glioblastomas and glioma cells. Isoform 1 and isoform 2 are expressed in melanoma, carcinoma and fibroblast cell lines.
翻译后修饰
N-glycosylated.; The N-terminus may be blocked.
亚细胞定位
Cell membrane. Cell surface.
UNIPROT
别名
170 kDa melanoma membrane bound gelatinase antibody
170 kDa melanoma membrane-bound gelatinase antibody
DPPIV antibody
FAP antibody
FAPA antibody
Fibroblast activation protein alpha antibody
Integral membrane serine protease antibody
SEPR_HUMAN antibody
Seprase antibody
图片
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mIHC analysis of human cervical cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-FAP antibody (IRS289RB) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
FAP Recombinant Rabbit Monoclonal Antibody [JA56-11] - BSA and Azide free
Application: WB,IHC-P,IHC-Fr
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
