Phospho-EGFR (Y992) Recombinant Rabbit Monoclonal Antibody [PSH23-63] - BSA and Azide free

RMB: 5600 特惠 5600

产品规格

100μg

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Catalog# HA751904

Phospho-EGFR (Y992) Recombinant Rabbit Monoclonal Antibody [PSH23-63] - BSA and Azide free

Application

WB
IF-Cell
FC

Reactivity

Human

With BSA and Azide

HA724388
含抗保成分

Conjugation

unconjugated

概述

产品名称

Phospho-EGFR (Y992) Recombinant Rabbit Monoclonal Antibody [PSH23-63] - BSA and Azide free

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic phosphopeptide corresponding to residues surrounding Tyr992 of human EGFR.

种属反应性

Human

验证应用

WB, IF-Cell, FC

分子量

Predicted band size: 134 kDa

阳性对照

A431 treated with 100ng/mL EGF for 30min cell lysate.

偶联

unconjugated

克隆号

PSH23-63

产品特性

形态

Liquid

浓度

批次浓度查询

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

PBS (pH7.4).

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

WB
1:2,000

IF-Cell
1:1,000

FC
1:10,000

靶点

功能

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is a transmembrane protein that is a receptor for members of the epidermal growth factor family (EGF family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). In many cancer types, mutations affecting EGFR expression or activity could result in cancer. Deficient signaling of the EGFR and other receptor tyrosine kinases in humans is associated with diseases such as Alzheimer's, while over-expression is associated with the development of a wide variety of tumors. Interruption of EGFR signalling, either by blocking EGFR binding sites on the extracellular domain of the receptor or by inhibiting intracellular tyrosine kinase activity, can prevent the growth of EGFR-expressing tumours and improve the patient's condition.

背景文献

1. Nakamura T et al. LRIG1 inhibits STAT3-dependent inflammation to maintain corneal homeostasis. J Clin Invest 124:385-97 (2014).

2. Furcht CM et al. Multivariate signaling regulation by SHP2 differentially controls proliferation and therapeutic response in glioma cells. J Cell Sci 127:3555-67 (2014).

亚细胞定位

Cell membrane, Nucleus membrane, Nucleus, Endoplasmic reticulum membrane, Golgi apparatus membrane, Endosome.

UNIPROT

SwissProt: P00533 Human

别名

Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody

Cell growth inhibiting protein 40 antibody

Cell proliferation inducing protein 61 antibody

EGF R antibody

EGFR antibody

EGFR_HUMAN antibody

Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody

Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody

Epidermal growth factor receptor antibody

erb-b2 receptor tyrosine kinase 1 antibody

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图片

☑ Cell treatment (CT)

Western blot analysis of Phospho-EGFR (Y992) on different lysates with Rabbit anti-Phospho-EGFR (Y992) antibody (HA751904) at 1/2,000 dilution.

Lane 1: A431 (Human epidermoid carcinoma skin squamous cells) cell lysate
Lane 2: A431 treated with 100ng/mL EGF for 30min cell lysate

Lysates/proteins at 20 µg/Lane.
Exposure time: 12 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751904, 1/2,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 134 kDa
Observed band size: 175 kDa
☑ Cell treatment (CT)

Application: Immunocytochemistry (IF-cell)

Species: Human
Sample: A431 (Human epidermoid carcinoma skin squamous cells) treated with EGF(100ng/ml 30min)

Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.

Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA751904, 1/1,000, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.

Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue).
☑ Cell treatment (CT)

Application: Flow Cytometry (Intra)

Species: Human
Sample: A431 (Human epidermoid carcinoma skin squamous cells) treated with EGF(100ng/ml 30min)

Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature.

Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA751904 (1/10,000) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Phospho-EGFR (Y992) Recombinant Rabbit Monoclonal Antibody [PSH23-63] - BSA and Azide free

RMB: 5600 特惠 5600

产品规格

联系我们

概述

产品名称

抗体类型

免疫原

种属反应性

验证应用

分子量

阳性对照

偶联

克隆号

产品特性

形态

浓度

存放说明

存储缓冲液

亚型

纯化方式

应用稀释度

靶点

功能

背景文献

亚细胞定位

UNIPROT

别名

图片

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Phospho-EGFR (Y992) Recombinant Rabbit Monoclonal Antibody [PSH23-63]

Application: WB,IF-Cell,FC