CD13 Recombinant Rabbit Monoclonal Antibody [SN71-04] - BSA and Azide free
Catalog# HA751849
CD13 Recombinant Rabbit Monoclonal Antibody [SN71-04] - BSA and Azide free
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WB
-
IHC-P
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IP
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IF-Tissue
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Human
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Mouse
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Rat
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ET1611-61
含抗保成分
-
unconjugated
概述
产品名称
CD13 Recombinant Rabbit Monoclonal Antibody [SN71-04] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human CD13 aa 401-450 / 967.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P, IP, IF-Tissue
分子量
Predicted band size: 110 kDa
阳性对照
293T cell lysate, PANC-1 cell lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate, human liver tissue.
偶联
unconjugated
克隆号
SN71-04
产品特性
形态
Liquid
浓度
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000-1:5,000
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IHC-P
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1:2,000
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IP
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1-2μg/sample
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IF-Tissue
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1:500
靶点
功能
CD13, or aminopeptidase N, is a type II transmembrane glycoprotein that is expressed on most cells of Myeloid origin, including monocytes, basophils, eosinophils, neutrophils and Myeloid leukemias. CD13 is also found on certain epithelial cells, fibroblasts and osteoclasts. CD13 acts as a zinc-binding metalloprotease that plays a role in digestion and may function in the inactivation of some regulatory peptides such as enkephalins. CD13 may play a role in the invasion of cancer cells by enhancing their invasive capacity and metastatic behavior. The activity of CD13 can be inactivated using specific inhibitors that evoke apoptosis of CD13-positive cancer cells. Basic fibroblast growth factor (bFGF) expression upregulates CD13 expression in human melanoma cells by activating both the Myeloid and the epithelial CD13 promoter.
背景文献
1. Cui SX et al. 13F-1, a novel 5-fluorouracil prodrug containing an Asn-Gly-Arg (NO2) COOCH3 tripeptide, inhibits human colonic carcinoma growth by targeting Aminopeptidase N (APN/CD13). Eur J Pharmacol 734:50-9 (2014).
2. H rdtner C et al. High glucose activates the alternative ACE2/Ang-(1-7)/Mas and APN/Ang IV/IRAP RAS axes in pancreatic -cells. Int J Mol Med 32:795-804 (2013).
序列相似性
Belongs to the peptidase M1 family.
组织特异性
Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients.
翻译后修饰
Sulfated.; N- and O-glycosylated.; May undergo proteolysis and give rise to a soluble form.
亚细胞定位
Cell membrane.
别名
Alanyl (membrane) aminopeptidase antibody
Alanyl aminopeptidase antibody
Aminopeptidase M antibody
Aminopeptidase N antibody
AMPN_HUMAN antibody
ANPEP antibody
AP M antibody
AP N antibody
AP-M antibody
AP-N antibody
展开图片
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☑ Knockdown (KD)
Western blot analysis of CD13 on different lysates with Rabbit anti-CD13 antibody (HA751849) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-CD13 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 110 kDa
Observed band size: 150 kDa
Exposure time: 110 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751849) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Relative expression (RE)
Western blot analysis of CD13 on different lysates with Rabbit anti-CD13 antibody (HA751849) at 1/2,000 dilution.
Lane 1: 293T cell lysate (negative) (20 µg/Lane)
Lane 2: PANC-1 cell lysate (5 µg/Lane)
Lane 3: Mouse kidney tissue lysate (20 µg/Lane)
Lane 4: Rat kidney tissue lysate (20 µg/Lane)
Predicted band size: 110 kDa
Observed band size: 150 kDa
Exposure time: 45 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751849) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-CD13 antibody (HA751849) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751849) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
CD13 was immunoprecipitated from 0.2 mg PANC-1 cell lysate with HA751849 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751849 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: PANC-1 cell lysate (input)
Lane 2: HA751849 IP in PANC-1 cell lysate
Lane 3: Rabbit IgG instead of HA751849 in PANC-1 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 20 seconds; ECL: K1801 -
Application: IF-Tissue
Species: Mouse
Site: kidney
Sample: Paraffin-embedded section
Antibody concentration: 1/500
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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