IRE1 Recombinant Rabbit Monoclonal Antibody [PSH10-60] - BSA and Azide free
Catalog# HA751363
IRE1 Recombinant Rabbit Monoclonal Antibody [PSH10-60] - BSA and Azide free
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WB
-
Human
-
Mouse
-
Rat
-
Monkey
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HA723225
含抗保成分
-
unconjugated
概述
产品名称
IRE1 Recombinant Rabbit Monoclonal Antibody [PSH10-60] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human IRE1 aa 1-400.
种属反应性
Human, Mouse, Rat, Monkey
验证应用
WB
分子量
Predicted band size: 110 kDa
阳性对照
HEK-293 cell lysate, Neuro-2a cell lysate, C6 cell lysate, COS-1 cell lysate.
偶联
unconjugated
克隆号
PSH10-60
产品特性
形态
Liquid
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:5,000
靶点
功能
The serine/threonine-protein kinase/endoribonuclease inositol-requiring enzyme 1 α (IRE1α) is an enzyme that in humans is encoded by the ERN1 gene. The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals (mainly the unfolded protein response). Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
背景文献
1. Huang R et al. IRE1 signaling regulates chondrocyte apoptosis and death fate in the osteoarthritis. J Cell Physiol. 2022 Jan
2. Grandjean JMD et al. Pharmacologic IRE1/XBP1s activation confers targeted ER proteostasis reprogramming. Nat Chem Biol. 2020 Oct
亚细胞定位
Endoplasmic reticulum membrane; nucleus.
别名
Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
Endoplasmic reticulum to nucleus signaling 1 antibody
Endoplasmic reticulum-to-nucleus signaling 1 antibody
Endoribonuclease antibody
ER to nucleus signaling 1 antibody
ERN 1 antibody
Ern1 antibody
ERN1_HUMAN antibody
hIRE 1p antibody
hIRE1p antibody
展开图片
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Western blot analysis of IRE1 on different lysates with Rabbit anti-IRE1 antibody (HA751363) at 1/5,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: Neuro-2a cell lysate
Lane 3: C6 cell lysate
Lane 4: COS-1 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 110 kDa
Observed band size: 110 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751363) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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IRE1 Recombinant Rabbit Monoclonal Antibody [PSH10-60]
Application: WB
Reactivity: Human,Mouse,Rat,Monkey
Conjugate: unconjugated
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