IRE1 Mouse Monoclonal Antibody [C9-F2]
Catalog# EM1708-88
IRE1 Mouse Monoclonal Antibody [C9-F2]
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WB
-
IHC-P
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Human
-
unconjugated
概述
产品名称
IRE1 Mouse Monoclonal Antibody [C9-F2]
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein
种属反应性
Human
验证应用
WB, IHC-P
分子量
110 kDa
阳性对照
Raji, A431, Jurkat, Hela, HEK293 cell lysate, human placenta tissue, human brain tissue, human stomach tissue.
偶联
unconjugated
克隆号
C9-F2
产品特性
形态
Liquid
存放说明
4℃; -20℃ for long term storage.
存储缓冲液
Purified antibody in PBS with 0.05% sodium azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:500-1:2,000
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IHC-P
-
1:50-1:200
靶点
功能
The accumulation of unfolded proteins within the endoplasmic recticulum (ER) of yeast and mammalian cells activates the unfolded protein response (UPR) pathway and leads to the transcription of ER-specific genes involved in protein folding. The activation of the UPR requires the ER transmembrane kinase IRE1p (for inositol-requiring and ER-to-nucleus signaling protein). IRE1α and IRE1β are two mammalian homologs of the yeast IRE1p. These related proteins localize to the ER lumen and contain both a short transmembrane domain that spans the ER membrane and a cytosolic Ser/Thr kinase domain. IRE1 activation involves the oligomerization and trans-phosphorylation of the cytosolic portion of the proteins, which then potentiates its intrinsic kinase activity and, in turn, stimulates transcription of UPR-targeted genes. In response to stress, sensors for the ER mammalian cells activate IRE1α and IRE1β, which then results in the phosphorylation of JNK (Jun N-Terminal Kinase) and the activation of the cellular MAP kinase pathway.
背景文献
暂无
亚细胞定位
Endoplasmic reticulum membrane.
UNIPROT
别名
Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
Endoplasmic reticulum to nucleus signaling 1 antibody
Endoplasmic reticulum-to-nucleus signaling 1 antibody
Endoribonuclease antibody
ER to nucleus signaling 1 antibody
ERN 1 antibody
Ern1 antibody
ERN1_HUMAN antibody
hIRE 1p antibody
hIRE1p antibody
展开图片
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Western blot analysis of ERN1 on different cell lysate using anti-ERN1 antibody at 1/1,000 dilution.
Positive control:
Line1: Raji
Line2: A431
Line3: Jurkat
Line4: Hela
Line5: HEK293 -
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ERN1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-ERN1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human stomach tissue using anti-ERN1 antibody. Counter stained with hematoxylin.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
IRE1 Recombinant Rabbit Monoclonal Antibody [PSH10-60]
Application: WB
Reactivity: Human,Mouse,Rat,Monkey
Conjugate: unconjugated
同靶点 & 同通路的产品
IRE1 Rabbit Polyclonal Antibody
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
IRE1 Recombinant Rabbit Monoclonal Antibody [PSH10-60]
Application: WB
Reactivity: Human,Mouse,Rat,Monkey
Conjugate: unconjugated
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