Glucose Transporter GLUT4 Recombinant Rabbit Monoclonal Antibody [PSH10-06] - BSA and Azide free
Catalog# HA751336
Glucose Transporter GLUT4 Recombinant Rabbit Monoclonal Antibody [PSH10-06] - BSA and Azide free
-
WB
-
IHC-Fr
-
Mouse
-
Rat
-
HA723175
含抗保成分
-
unconjugated
概述
产品名称
Glucose Transporter GLUT4 Recombinant Rabbit Monoclonal Antibody [PSH10-06] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within human GLUT4 aa 447-509.
种属反应性
Mouse, Rat
验证应用
WB, IHC-Fr
分子量
Predicted band size: 55 kDa
阳性对照
Mouse heart tissue, rat skeletal muscle tissue, Mouse heart tissue lysate, Mouse skeletal muscle tissue lysate, Rat skeletal muscle tissue lysate.
偶联
unconjugated
克隆号
PSH10-06
产品特性
形态
Liquid
浓度
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:5,000
-
IHC-Fr
-
1:500
靶点
功能
Glucose transporter type 4 (GLUT4), also known as solute carrier family 2, facilitated glucose transporter member 4, is a protein encoded, in humans, by the SLC2A4 gene. GLUT4 is the insulin-regulated glucose transporter found primarily in adipose tissues and striated muscle (skeletal and cardiac). At the cell surface, GLUT4 permits the facilitated diffusion of circulating glucose down its concentration gradient into muscle and fat cells. Once within cells, glucose is rapidly phosphorylated by glucokinase in the liver and hexokinase in other tissues to form glucose-6-phosphate, which then enters glycolysis or is polymerized into glycogen. Glucose-6-phosphate cannot diffuse back out of cells, which also serves to maintain the concentration gradient for glucose to passively enter cells.
背景文献
1. van Gerwen J et al. Insulin signalling and GLUT4 trafficking in insulin resistance. Biochem Soc Trans. 2023 Jun
2. Yuan Y et al. Cryo-EM structure of human glucose transporter GLUT4. Nat Commun. 2022 May
亚细胞定位
Cell membrane, Endomembrane system, Cytoplasm, perinuclear region.
别名
insulin-responsive antibody
Glucose transporter GLUT 4 antibody
Glucose transporter type 4 antibody
Glucose transporter type 4 insulin responsive antibody
GLUT 4 antibody
GLUT-4 antibody
GLUT4 antibody
GTR4_HUMAN antibody
Insulin responsive glucose transporter type 4 antibody
kug antibody
展开图片
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Immunofluorescence analysis of frozen mouse heart tissue with Rabbit anti-Glucose Transporter GLUT4 antibody (HA751336) at 1/500 dilution.
The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA751336, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunofluorescence analysis of frozen rat skeletal muscle tissue with Rabbit anti-Glucose Transporter GLUT4 antibody (HA751336) at 1/500 dilution.
The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA751336, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
☑ Relative expression (RE)
Western blot analysis of Glucose Transporter GLUT4 on different lysates with Rabbit anti-Glucose Transporter GLUT4 antibody (HA751336) at 1/5,000 dilution.
Lane 1: Mouse heart tissue lysate (no heat) (30 µg/Lane)
Lane 2: Mouse skeletal muscle tissue lysate (no heat) (30 µg/Lane)
Lane 3: Mouse kidney tissue lysate (no heat) (negative) (30 µg/Lane)
Lane 4: Rat kidney tissue lysate (negative) (30 µg/Lane)
Lane 5: Rat lung tissue lysate (no heat) (negative) (30 µg/Lane)
Lane 6: Rat skeletal muscle tissue lysate (no heat) (30 µg/Lane)
Notice: no heat means the lysate is not boiled.
Predicted band size: 55 kDa
Observed band size: 50 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751336) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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