CD4 Recombinant Rabbit Monoclonal Antibody [PSH08-22] - BSA and Azide free
Rmb: 5600
Catalog# HA751213
CD4 Recombinant Rabbit Monoclonal Antibody [PSH08-22] - BSA and Azide free
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WB
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IF-Cell
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IHC-P
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IHC-Fr
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Mouse
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HA722966
含抗保成分
-
unconjugated
概述
产品名称
CD4 Recombinant Rabbit Monoclonal Antibody [PSH08-22] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within mouse CD4 aa 27-394.
种属反应性
Mouse
验证应用
WB, IF-Cell, IHC-P, IHC-Fr
靶点分子量
Predicted band size: 51 kDa
阳性对照
Mouse spleen tissue lysate, Mouse thymus tissue lysates, mouse splenocytes, mouse spleen tissue, mouse thymus tissue.
偶联
unconjugated
克隆号
PSH08-22
产品特性
形态
Liquid
浓度
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:2,000
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IF-Cell
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1:250
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IHC-P
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1:1,000 (IF-Tissue is not recommended)
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IHC-Fr
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1:500
靶点
功能
In molecular biology, CD4 (cluster of differentiation 4) is a glycoprotein that serves as a co-receptor for the T-cell receptor (TCR). CD4 is found on the surface of immune cells such as helper T cells, monocytes, macrophages, and dendritic cells. It was discovered in the late 1970s and was originally known as leu-3 and T4 (after the OKT4 monoclonal antibody that reacted with it) before being named CD4 in 1984. In humans, the CD4 protein is encoded by the CD4 gene. CD4+ T helper cells are white blood cells that are an essential part of the human immune system. They are often referred to as CD4 cells, T helper cells or T4 cells. They are called helper cells because one of their main roles is to send signals to other types of immune cells, including CD8 killer cells, which then destroy the infectious particle. If CD4 cells become depleted, for example in untreated HIV infection, or following immune suppression prior to a transplant, the body is left vulnerable to a wide range of infections that it would otherwise have been able to fight.
背景文献
1. Ruterbusch M et al. In Vivo CD4(+) T Cell Differentiation and Function: Revisiting the Th1/Th2 Paradigm. Annu Rev Immunol. 2020 Apr
2. Oh DY et al. Cytotoxic CD4(+) T cells in cancer: Expanding the immune effector toolbox. Immunity. 2021 Dec
亚细胞定位
Cell membrane.
UNIPROT
别名
CD 4 antibody
CD4 (L3T4) antibody
CD4 antibody
CD4 antigen (p55) antibody
CD4 antigen antibody
CD4 molecule antibody
CD4 receptor antibody
CD4+ Lymphocyte deficiency, included antibody
CD4_HUMAN antibody
CD4mut antibody
展开图片
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☑ Relative expression (RE)
Western blot analysis of CD4 on different lysates with Rabbit anti-CD4 antibody (HA751213) at 1/2,000 dilution.
Lane 1: C2C12 cell lysate (negative) (20 µg/Lane)
Lane 2: Mouse spleen tissue lysate (40 µg/Lane)
Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time: 30 seconds; ECL: K18012;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751213) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of CD4 on Mouse thymus tissue lysates with Rabbit anti-CD4 antibody (HA751213) at 1/2,000 dilution.
Lysates/proteins at 40 µg/Lane.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751213) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of mouse splenocytes labeling CD4 with Rabbit anti-CD4 antibody (HA751213) at 1/250 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD4 antibody (HA751213) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CD4 antibody (HA751213) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751213) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse thymus tissue with Rabbit anti-CD4 antibody (HA751213) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751213) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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