beta Amyloid 1-40 Recombinant Rabbit Monoclonal Antibody [PSH05-86] - BSA and Azide free
Catalog# HA751034
beta Amyloid 1-40 Recombinant Rabbit Monoclonal Antibody [PSH05-86] - BSA and Azide free
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WB
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IHC-P
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IF-Tissue
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Human
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Mouse
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HA722485
含抗保成分
-
unconjugated
概述
产品名称
beta Amyloid 1-40 Recombinant Rabbit Monoclonal Antibody [PSH05-86] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human beta Amyloid 1-40 peptide.
种属反应性
Human, Mouse
验证应用
WB, IHC-P, IF-Tissue
分子量
Predicted band size: 4 kDa
阳性对照
APP/PS1, 6-month mouse of AD brain tissue.
偶联
unconjugated
克隆号
PSH05-86
产品特性
形态
Liquid
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
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1:1,000
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IHC-P
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1:1,000
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IF-Tissue
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1:200
靶点
功能
This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene.
背景文献
1. Janelidze S et al. Head-to-Head Comparison of 8 Plasma Amyloid-β 42/40 Assays in Alzheimer Disease. JAMA Neurol. 2021 Nov
亚细胞定位
Cell membrane, Membrane, Perikaryon, Cell projection, growth cone, Membrane, clathrin-coated pit, Early endosome, Cytoplasmic vesicle.
别名
A4 antibody
A4_HUMAN antibody
AAA antibody
ABETA antibody
ABPP antibody
AICD-50 antibody
AICD-57 antibody
AICD-59 antibody
AID(50) antibody
AID(57) antibody
展开图片
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Western blot analysis of beta Amyloid 1-40 on different peptides with Rabbit anti-beta Amyloid 1-40 antibody (HA751034) at 1/1,000 dilution.
Lane 1: Human Aβ37 full length peptide (negative)
Lane 2: Human Aβ38 full length peptide (negative)
Lane 3: Human Aβ39 full length peptide (negative)
Lane 4: Human Aβ40 full length peptide (positive)
Lane 5: Human Aβ41 full length peptide (negative)
Lane 6: Human Aβ42 full length peptide (negative)
Lane 7: Human Aβ43 full length peptide (negative)
Lane 8: Mouse Aβ40 full length peptide (positive)
Lysates/proteins at 100 ng/Lane.
Predicted band size: 4 kDa
Observed band size: 4 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751034) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded APP/PS1, 6-month mouse of AD brain tissue with Rabbit anti-beta Amyloid 1-40 antibody (HA751034) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751034) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded normal mouse brain tissue (negative control) with Rabbit anti-beta Amyloid 1-40 antibody (HA751034) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751034) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunofluorescence analysis of paraffin-embedded APP/PS1, 6-month mouse of AD brain tissue labeling beta Amyloid 1-40 with Rabbit anti-beta Amyloid 1-40 antibody (HA751034) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA751034, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
☑ Relative expression (RE)
Immunofluorescence analysis of paraffin-embedded normal mouse brain tissue (negative control) labeling beta Amyloid 1-40 with Rabbit anti-beta Amyloid 1-40 antibody (HA751034) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA751034, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
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