Steroid sulfatase Recombinant Rabbit Monoclonal Antibody [PSH05-74] - BSA and Azide free
Catalog# HA751024
Steroid sulfatase Recombinant Rabbit Monoclonal Antibody [PSH05-74] - BSA and Azide free
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WB
-
IHC-P
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Human
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HA722441
含抗保成分
-
unconjugated
概述
产品名称
Steroid sulfatase Recombinant Rabbit Monoclonal Antibody [PSH05-74] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human Steroid sulfatase aa 22-184 and aa 235-583.
种属反应性
Human
验证应用
WB, IHC-P
分子量
Predicted band size: 65 kDa
阳性对照
Jurkat cell lysate HepG2 cell lysate, MCF7 cell lysate, Huh7 cell lysate, T-47D cell lysate, human placenta tissue.
偶联
unconjugated
克隆号
PSH05-74
产品特性
形态
Liquid
浓度
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:2,000
-
IHC-P
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1:3,000
靶点
功能
Catalyzes the conversion of sulfated steroid precursors, such as dehydroepiandrosterone sulfate (DHEA-S) and estrone sulfate to the free steroid. Defects in STS are the cause of ichthyosis X-linked (IXL) [MIM:308100]. Ichthyosis X-linked is a keratinization disorder manifesting with mild erythroderma and generalized exfoliation of the skin within a few weeks after birth. Affected boys later develop large, polygonal, dark brown scales, especially on the neck, extremities, trunk, and buttocks.
背景文献
1. Oyama N., Satoh M., Iwatsuki K., Kaneko F. Novel point mutations in the steroid sulfatase gene in patients with X- linked ichthyosis: transfection analysis using the mutated genes. J. Invest. Dermatol. 114:1195-1199 (2000)
2. Matsumoto J., Ariyoshi N., Ishii I., Kitada M. Functional characterization of seven single-nucleotide polymorphisms of the steroid sulfatase gene found in a Japanese population. J. Hum. Genet. 58:267-272 (2013)
亚细胞定位
Cytoplasmic vesicle. Endoplasmic reticulum.
UNIPROT
别名
ARSC antibody
ARSC1 antibody
Arylsulfatase C antibody
Arylsulfatase C isozyme S antibody
ASC antibody
EC 3.1.6.2 antibody
ES antibody
Estrone sulfatase antibody
SSDD antibody
Steroid sulfatase (microsomal) antibody
展开图片
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☑ Relative expression (RE)
Western blot analysis of Steroid sulfatase on different lysates with Rabbit anti-Steroid sulfatase antibody (HA751024) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: LNCaP cell lysate (negative)
Lane 3: HepG2 cell lysate
Lane 4: MCF7 cell lysate
Lane 5: Huh7 cell lysate
Lane 6: T-47D cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 65 kDa
Observed band size: 65 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751024) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Steroid sulfatase antibody (HA751024) at 1/3,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751024) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded human pancreas tissue (negative) with Rabbit anti-Steroid sulfatase antibody (HA751024) at 1/3,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751024) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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