RIP3 Recombinant Rabbit Monoclonal Antibody [PSH04-79] - BSA and Azide free

☑ Relative expression (RE)

This data was developed using HA722183, the same antibody clone in a different buffer formulation.
Western blot analysis of RIP3 on different lysates with Rabbit anti-RIP3 antibody (HA722183) at 1/2,000 dilution.

Lane 1: THP-1 cell lysate (30 µg/Lane)
Lane 2: HT-29 cell lysate (30 µg/Lane)
Lane 3: SK-Br-3 cell lysate (30 µg/Lane)
Lane 4: HeLa cell lysate (negative) (30 µg/Lane)

Predicted band size: 57 kDa
Observed band size: 55 kDa

Exposure time: 1 minute; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722183) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

☑ Relative expression (RE)

This data was developed using HA722183, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded HT-29 (positive) and HeLa (negative) cells with Rabbit anti-RIP3 antibody (HA722183) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722183) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

This data was developed using HA722183, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-RIP3 antibody (HA722183) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722183) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.