RIP3 Recombinant Rabbit Monoclonal Antibody [PSH04-78]
Catalog# HA722182
RIP3 Recombinant Rabbit Monoclonal Antibody [PSH04-78]
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WB
-
IHC-P
-
Human
概述
产品名称
RIP3 Recombinant Rabbit Monoclonal Antibody [PSH04-78]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within human RIP3 aa 401-450 / 518.
种属反应性
Human
验证应用
WB, IHC-P
分子量
Predicted band size: 57 kDa
阳性对照
THP-1 cell lysate, HT-29 cell lysate, SK-Br-3 cell lysate, HT-29, human colon cancer tissue.
偶联
unconjugated
克隆号
PSH04-78
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:2,000
-
IHC-P
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1:2,000-1:5,000
靶点
功能
Receptor-interacting serine/threonine-protein kinase 3 is an enzyme that is encoded by the RIPK3 gene in humans. The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. It contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce necroptosis by interaction with RIPK1 and MLKL in a protein complex termed the necrosome. Interactions between RIPK1 and RIPK3 also form a necrosome, which triggers apoptosis.
背景文献
1. Zeng X et al. Activated Drp1 regulates p62-mediated autophagic flux and aggravates inflammation in cerebral ischemia-reperfusion via the ROS-RIP1/RIP3-exosome axis. Mil Med Res. 2022 May
2. Chen X et al. Mosaic composition of RIP1-RIP3 signalling hub and its role in regulating cell death. Nat Cell Biol. 2022 Apr
亚细胞定位
Cytoplasm, cytosol, Nucleus.
UNIPROT
别名
Receptor interacting protein 3 antibody
Receptor interacting serine threonine kinase 3 antibody
Receptor interacting serine/threonine protein kinase 3 antibody
Receptor-interacting protein 3 antibody
Receptor-interacting serine/threonine-protein kinase 3 antibody
RIP 3 antibody
RIP like protein kinase 3 antibody
RIP-3 antibody
RIP-like protein kinase 3 antibody
RIPK 3 antibody
展开图片
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☑ Relative expression (RE)
Western blot analysis of RIP3 on different lysates with Rabbit anti-RIP3 antibody (HA722182) at 1/2,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: HT-29 cell lysate
Lane 3: SK-Br-3 cell lysate
Lane 4: HeLa cell lysate (negative)
Lysates/proteins at 30 µg/Lane.
Predicted band size: 57 kDa
Observed band size: 55 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722182) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded HT-29 (positive) and HeLa (negative) cells with Rabbit anti-RIP3 antibody (HA722182) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722182) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-RIP3 antibody (HA722182) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722182) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Reactivity: Human
Conjugate: unconjugated
