RIP3 Rabbit Polyclonal Antibody
Catalog# ER1902-67
RIP3 Rabbit Polyclonal Antibody
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WB
-
IHC-P
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FC
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Human
概述
产品名称
RIP3 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Synthetic peptide within Human RIP3 aa 380-420.
种属反应性
Human
验证应用
WB, IHC-P, FC
分子量
Predicted band size: 57 kDa
阳性对照
Human lung carcinoma tissue lysate, human placenta tissue lysate , SW620, HT-29 cell lysates.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
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WB
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1:500-1:2,000
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
发表文章中的应用
| WB | 查看 2 篇文献如下 |
发表文章中的种属
| Human | 查看 1 篇文献如下 |
| Mouse | 查看 1 篇文献如下 |
靶点
功能
Receptor-interacting serine/threonine-protein kinase 3 is an enzyme that in humans is encoded by the RIPK3 gene. The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. Essential for necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production.
背景文献
1. Liu Y. et. al. RIP1/RIP3-regulated necroptosis as a target for multifaceted disease therapy (Review). Int J Mol Med. 2019 Sep
2. Zhang T. et. al. CaMKII is a RIP3 substrate mediating ischemia- and oxidative stress-induced myocardial necroptosis. Nat Med. 2016 Feb
序列相似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
组织特异性
Highly expressed in the pancreas. Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers.
翻译后修饰
RIPK1 and RIPK3 undergo reciprocal auto- and trans-phosphorylation. Phosphorylation of Ser-199 plays a role in the necroptotic function of RIPK3. Phosphorylation at Ser-227 is required for binding MLKL. Phosphorylation at Thr-182 is important for its kinase activity, interaction with PELI1 and PELI1-mediated 'Lys-48'-linked polyubiquitination and for its ability to mediate TNF-induced necroptosis.; Polyubiquitinated with 'Lys-48' and 'Lys-63'-linked chains by BIRC2/c-IAP1 and BIRC3/c-IAP2, leading to activation of NF-kappa-B. Polyubiquitinated with 'Lys-48'-linked chains by PELI1 leading to its subsequent proteasome-dependent degradation. Ubiquitinated by STUB1 leading to its subsequent proteasome-dependent degradation.
亚细胞定位
Cytoplasm, cytosol, Nucleus.
别名
Receptor interacting protein 3 antibody
Receptor interacting serine threonine kinase 3 antibody
Receptor interacting serine/threonine protein kinase 3 antibody
Receptor-interacting protein 3 antibody
Receptor-interacting serine/threonine-protein kinase 3 antibody
RIP 3 antibody
RIP like protein kinase 3 antibody
RIP-3 antibody
RIP-like protein kinase 3 antibody
RIPK 3 antibody
展开图片
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Western blot analysis of RIP3 on HT-29 cell lysates with Rabbit anti-RIP3 antibody (ER1902-67) at 1/1,000 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 57 kDa
Observed band size: 50 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1902-67) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of RIP3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-67, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: human lung carcinoma tissue lysate
Lane 2: human placenta tissue lysate -
Flow cytometric analysis of RIP3 was done on SW620 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-67, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Aurantiamide mitigates acute kidney injury by suppressing renal necroptosis and inflammation via GRPR-dependent mechanism
Author: He Ruobing,et al
PMID: 39059099
应用: WB
反应种属: Human
发表时间: 2024 Aug
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Citation
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Selumetinib - a potential small molecule inhibitor for osteoarthritis treatment
Author: Zheng, X., Qiu, J., Pan, W., Gong, Y., Zhang, W., Jiang, T., Chen, L., Chen, W., & Hong, Z.
PMID: 36238555
应用: WB
反应种属: Mouse
发表时间: 2022 Sept
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Citation
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Conjugate: unconjugated
