MBD1 Recombinant Rabbit Monoclonal Antibody [PSH01-78] - BSA and Azide free
Catalog# HA750752
MBD1 Recombinant Rabbit Monoclonal Antibody [PSH01-78] - BSA and Azide free
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WB
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Human
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Mouse
-
Rat
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HA721730
含抗保成分
-
unconjugated
概述
产品名称
MBD1 Recombinant Rabbit Monoclonal Antibody [PSH01-78] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within mouse MBD1 aa 1-200 / 636.
种属反应性
Human, Mouse, Rat
验证应用
WB
分子量
Predicted band size: 67 kDa
阳性对照
HeLa cell lysate, 293T cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, human kidney tissue lysate, mouse kidney tissue lysate, mouse brain tissue lysate, mouse testis tissue lysate, mouse lung tissue lysate, rat brain tissue lysate, rat testis tissue lysate.
偶联
unconjugated
克隆号
PSH01-78
产品特性
形态
Liquid
浓度
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
靶点
功能
MBD1 is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains multiple domains: MBD at the N-terminus that functions both in binding to methylated DNA and in protein interactions; several CXXC-type zinc finger domains that mediate binding to non-methylated CpG dinucleotides; transcriptional repression domain (TRD) at the C-terminus that is involved in transcription repression and in protein interactions. Numerous alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
背景文献
1. Wensheng L, Bo Z, Qiangsheng H, et al. MBD1 promotes the malignant behavior of gallbladder cancer cells and induces chemotherapeutic resistance to gemcitabine. Cancer Cell Int. 2019 Sep 9; 19:232.
2. Lyst MJ, Nan X, Stancheva I. Retraction: Regulation of MBD1-mediated transcriptional repression by SUMO and PIAS proteins. EMBO J. 2019 Oct 15;38(20): e103220.
亚细胞定位
Nucleus, Nucleus matrix, Nucleus speckle, Chromosome
别名
CXXC 3 antibody
CXXC-type zinc finger protein 3 antibody
CXXC3 antibody
MBD 1 antibody
Mbd1 antibody
MBD1_HUMAN antibody
MECP1 COMPLEX antibody
Methyl CpG binding domain protein 1 antibody
Methyl CpG binding domain protein 1 isoform PCM1 antibody
Methyl CpG binding protein MBD1 antibody
展开图片
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Western blot analysis of MBD1 on different lysates with Rabbit anti-MBD1 antibody (HA750752) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: C2C12 cell lysate
Lane 5: Human kidney tissue lysate
Lane 6: Mouse kidney tissue lysate
Lane 7: Mouse brain tissue lysate
Lane 8: Mouse testis tissue lysate
Lane 9: Mouse lung tissue lysate
Lane 10: Rat brain tissue lysate
Lane 11: Rat testis tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 67 kDa
Observed band size: 67 kDa
Exposure time: 2 minutes 18 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750752) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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