GST Recombinant Rabbit Monoclonal Antibody [SN70-05] - BSA and Azide free
Catalog# HA750257
GST Recombinant Rabbit Monoclonal Antibody [SN70-05] - BSA and Azide free
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WB
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IF-Cell
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IP
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Species independent
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ET1611-47
含抗保成分
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unconjugated
概述
产品名称
GST Recombinant Rabbit Monoclonal Antibody [SN70-05] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
GST tag recombinant protein.
种属反应性
Species independent
验证应用
WB, IF-Cell, IP
阳性对照
293T transfected with GST cell lysate, 293T transfected with GST-tagged Histone H3.1 cell lysate, HeLa transfected with GST-tagged Histone H3.1 cells.
偶联
unconjugated
克隆号
SN70-05
产品特性
形态
Liquid
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000-1:50,000
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IF-Cell
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1:50-1:200
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IP
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Use at an assay dependent concentration.
靶点
功能
The glutathione S-transferase (GST) family of enzymes comprises a long list of cytosolic, mitochondrial, and microsomal proteins that are capable of multiple reactions with a multitude of substrates, both endogenous and xenobiotic. Mammalian cytosolic GSTs are dimeric both subunits being from the same class of GSTs. The monomers are in the range of 22–29 kDa. Glutathione S-transferase is used to create the “GST gene fusion system” in genetic engineering. Here, GST is used to purify and detect proteins of interest.
背景文献
1. Pettit MW et al. Construction and physiochemical characterisation of a multi-composite, potential oral vaccine delivery system (VDS). Int J Pharm 468:264-71 (2014).
2. Zhang D et al. The Chromatin-Remodeling Factor PICKLE Integrates Brassinosteroid and Gibberellin Signaling during Skotomorphogenic Growth in Arabidopsis. Plant Cell 26:2472-2485 (2014).
别名
Glutathione S Transferase antibody
Glutathione S-transferase class-mu 26 kDa isozyme antibody
GST antibody
Sj26 antigen antibody
SjGST antibody
图片
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Western blot analysis of GST on different lysates with Rabbit anti-GST antibody (HA750257) at 1/50,000 dilution.
Lane 1: 293T transfected with GST cell lysate (2.5 µg/Lane)
Lane 2: 293T transfected with GST-tagged Histone H3.1 cell lysate (15 µg/Lane)
Exposure time: 46 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750257) at 1/50,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
GST was immunoprecipitated in 0.2mg 293T-OE-GST cell lysate with HA750257 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750257 at 1/50,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T transfected with GST-tagged Histone H3.1 cell lysate (input)
Lane 2: Rabbit IgG instead of HA750257 in 293T transfected with GST-tagged Histone H3.1 cell lysate
Lane 3: HA750257 IP in 293T transfected with GST-tagged Histone H3.1 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 24 seconds -
Immunocytochemistry analysis of HeLa transfected with GST-tagged Histone H3.1 cells labeling GST with Rabbit anti-GST antibody (HA750257) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GST antibody (HA750257) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Histone H3 (HA601278, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"