Tryptophan Hydroxylase 1 (TPH1) Recombinant Rabbit Monoclonal Antibody [SC53-07] - BSA and Azide free
Rmb: 5600
Catalog# HA750214
Tryptophan Hydroxylase 1 (TPH1) Recombinant Rabbit Monoclonal Antibody [SC53-07] - BSA and Azide free
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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IP
-
FC
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Human
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Mouse
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Rat
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ET1610-37
含抗保成分
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Cynomolgus monkey
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Pig
预测的种属支持售后
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unconjugated
概述
产品名称
Tryptophan Hydroxylase 1 (TPH1) Recombinant Rabbit Monoclonal Antibody [SC53-07] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human TPH1 aa 372-419 / 444.
种属反应性
Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
预测的种属支持售后
验证应用
WB, IF-Cell, IF-Tissue, IHC-P, IP, FC
靶点分子量
Predicted band size: 51 kDa
阳性对照
MDA-MB-231 cell lysate, LNCaP cell lysate, HL-60 cell lysate, THP-1 cell lysate, MCF7 cell lysate, human brain tissue, rat brain tissue, mouse cerebellum tissue, Hela.
偶联
unconjugated
克隆号
SC53-07
产品特性
形态
Liquid
浓度
存放说明
Store at 2-8℃. Avoid freeze.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-1:1,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
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IP
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Use at an assay dependent concentration.
靶点
功能
Phenylalanine hydroxylase (PAH), tyrosine hydroxylase (TH) and tryptophan hydroxylase (TPH) comprise a small family of monooxygenases that use tetrahydropterine as a cofactor during the catabolism of aromatic L-amino acids. PAH, TH and TPH all contain catalytic domains with an amino-terminal regulatory domain and a short carboxy-terminal tetramerization domain. Each of these enzymes also contains a single ferrous iron atom, which is bound to two histidines and a glutamate and is likely to be involved in the formation of the hydroxylating intermediate. TPH is the first and rate-limiting step in the biosynthesis of serotonin in the central nervous system and melatonin in the pineal gland. Alteration of TPH function may be a key factor in the pathology of several neuropsychiatric disorders associated with serotonin, including depression, aggression, alcoholism and schizophrenia. For instance, L-DOPA, which is used as a common therapy for Parkinson’s disease (PD) patients, inhibits TPH function, which subsequently, is thought to contribute to the onset of depression in PD patients.
背景文献
1. Cheng HH et al. Quiescent and proliferative fibroblasts exhibit differential p300 HAT activation through control of 5-methoxytryptophan production. PLoS One 9:e88507 (2014).
2. Dempsie Y et al. Dexfenfluramine and the oestrogen-metabolizing enzyme CYP1B1 in the development of pulmonary arterial hypertension. Cardiovasc Res 99:24-34 (2013).
序列相似性
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
组织特异性
Isoform 2 seems to be less widely expressed than isoform 1.
亚细胞定位
Cytosol, neuron projection.
别名
Indoleacetic acid 5 hydroxylase antibody
L tryptophan hydroxylase antibody
MGC119994 antibody
TPH 1 antibody
TPH antibody
TPH1 antibody
TPH1_HUMAN antibody
TPRH antibody
TRPH antibody
Tryptophan 5 hydroxylase 1 antibody
展开图片
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Western blot analysis of Tryptophan Hydroxylase 1 (TPH1) on different lysates with Rabbit anti-Tryptophan Hydroxylase 1 (TPH1) antibody (HA750214) at 1/1,000 dilution.
Lane 1: MDA-MB-231 cell lysate
Lane 2: LNCaP cell lysate
Lane 3: HL-60 cell lysate
Lane 4: THP-1 cell lysate
Lane 5: MCF7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 51 kDa
Observed band size: 72 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750214) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of TPH1 on different lysates with Rabbit anti-TPH1 antibody (HA750214) at 1/1,000 dilution.
Lane 1: MDA-MB-231-si NT cell lysate (10 µg/Lane)
Lane 2: MDA-MB-231-si TPH1 cell lysate (10 µg/Lane)
Predicted band size: 51 kDa
Observed band size: 72 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
ET1610-37 was shown to specifically react with TPH1 in MDA-MB-231-si NT cells. Weakened band was observed when MDA-MB-231-si TPH1 sample was tested. MDA-MB-231-si NT and Hela-si TPH1 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1610-37, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Tryptophan Hydroxylase 1 (TPH1) antibody (HA750214) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750214) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Tryptophan Hydroxylase 1 (TPH1) antibody (HA750214) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750214) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-Tryptophan Hydroxylase 1 (TPH1) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750214, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Flow cytometric analysis of Tryptophan Hydroxylase 1 (TPH1) was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (HA750214, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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