ROCK1 Recombinant Rabbit Monoclonal Antibody [ST05-19] - BSA and Azide free
Catalog# HA750181
ROCK1 Recombinant Rabbit Monoclonal Antibody [ST05-19] - BSA and Azide free
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WB
-
IF-Tissue
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IHC-P
-
IP
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Human
-
Mouse
-
Rat
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ET1609-53
含抗保成分
-
unconjugated
概述
产品名称
ROCK1 Recombinant Rabbit Monoclonal Antibody [ST05-19] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human ROCK1 aa aa 1104-1149 / 1,354.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Tissue, IHC-P, IP
分子量
Predicted band size: 158 kDa
阳性对照
A431 cell lysate, Human lung tissue lysate, Mouse spleen tissue lysate, C6 cell lysate, L6 cell lysate, human thyroid tissue, mouse thyroid tissue, rat thyroid tissue.
偶联
unconjugated
克隆号
ST05-19
产品特性
形态
Liquid
浓度
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
-
1:1,000-1:5,000
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IF-Tissue
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1:50
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IHC-P
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1:50-1:200
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IP
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Use at an assay dependent concentration.
靶点
功能
Rho, the Ras-related small GTPase, is responsible for the regulation of Actin-based cytoskeletal structures including stress fibers, focal adhesions and the contractile ring apparatus. Rho proteins function as molecular switches that are able to turn cytokinesis on and off. Although little is known about signaling downstream of Rho, a host of putative Rho effector proteins have been described, including rhophilin, Rhotekin, citron and the serine/threonine kinase, protein kinase N. Two additional Rho-activated serine/threonine kinases have been described, designated Rock-1 and Rock-2 (also referred to as Roka, for Rho-associated coil-containing protein kinase). Rock-1 and Rock-2 share a structural similarity with myotonic dystrophy kinase.
背景文献
1. Gentry EG et al. Rho Kinase Inhibition as a Therapeutic for Progressive Supranuclear Palsy and Corticobasal Degeneration. J Neurosci 36:1316-23 (2016).
2. Cai SD et al. MicroRNA-144 inhibits migration and proliferation in rectal cancer by downregulating ROCK-1. Mol Med Rep 12:7396-402 (2015).
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
组织特异性
Detected in blood platelets.
翻译后修饰
Autophosphorylated on serine and threonine residues.; Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.
亚细胞定位
Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane.
别名
coiled-coil-containing protein kinase 1 antibody
coiled-coil-containing protein kinase I antibody
MGC131603 antibody
MGC43611 antibody
p160 Rhoassociated coiled coil-forming protein kinase antibody
p160 ROCK-1 antibody
p160 ROCK1 antibody
p160ROCK antibody
PRO0435 antibody
Renal carcinoma antigen NY REN 35 antibody
展开图片
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Western blot analysis of ROCK1 on different lysates with Rabbit anti-ROCK1 antibody (HA750181) at 1/1,000 dilution.
Lane 1: A431 cell lysate (20 µg/Lane)
Lane 2: Human lung tissue lysate (20 µg/Lane)
Lane 3: Mouse spleen tissue lysate (20 µg/Lane)
Lane 4: C6 cell lysate (20 µg/Lane)
Lane 5: L6 cell lysate (20 µg/Lane)
Predicted band size: 158 kDa
Observed band size: 158 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750181) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Rabbit anti-ROCK1 antibody (HA750181) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750181) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse thyroid tissue with Rabbit anti-ROCK1 antibody (HA750181) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750181) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat thyroid tissue with Rabbit anti-ROCK1 antibody (HA750181) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750181) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
ROCK1 Recombinant Rabbit Monoclonal Antibody [ST05-19]
Application: WB,IF-Tissue,IHC-P,IP
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
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