V5 tag Recombinant Rabbit Monoclonal Antibody [SY30-01] - BSA and Azide free
Catalog# HA750086
V5 tag Recombinant Rabbit Monoclonal Antibody [SY30-01] - BSA and Azide free
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WB
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IF-Cell
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IP
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Species independent
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ET1605-41
含抗保成分
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unconjugated
概述
产品名称
V5 tag Recombinant Rabbit Monoclonal Antibody [SY30-01] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide immune sequence is CGKPIPNPLLGLDST.
种属反应性
Species independent
验证应用
WB, IF-Cell, IP
阳性对照
V5 tagged recombinant protein.
偶联
unconjugated
克隆号
SY30-01
产品特性
形态
Liquid
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:20,000-1:50,000
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IF-Cell
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1:2,000
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IP
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1-2μg/sample
靶点
功能
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The anti-V5 antibody detects recombinant proteins containing the 14 amino acid GKPIPNPLLGLDST sequence.
背景文献
1. Tatenhorst L et al. Fasudil attenuates aggregation of a-synuclein in models of Parkinson\'s disease. Acta Neuropathol Commun 4:39 (2016).
2. Ji H et al. EGFR phosphorylates FAM129B to promote Ras activation. Proc Natl Acad Sci U S A 113:644-9 (2016).
别名
GKPIPNPLLGLDST epitope tag antibody
GKPIPNPLLGLDST tag antibody
Protein Rev antibody
Regulator of expression of viral proteins antibody
rev antibody
V5 epitope tag antibody
图片
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Western blot analysis of V5 tag on different lysates with Rabbit anti-V5 tag antibody (HA750086) at 1/20,000 dilution.
Lane 1: 293T cell lysate
Lane 2: 293T transfected with V5-tagged Histone H3.1 (N-terminal) cell lysate
Lane 3: 293T transfected with V5-tagged Nanos homolog 3 (C-terminal) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750086) at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HeLa cells labeling V5 tag with Rabbit anti-V5 tag antibody (HA750086) at 1/2,000 dilution.
HeLa cells, transfected with V5-tagged empty control, Histone H3 (N-terminal) or Nanos homolog 3 (C-terminal) expression vector, respectively, were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-V5 tag antibody (HA750086) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
V5 tag (M1008-2, red) was stained at 1/1,000 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
V5 tag was immunoprecipitated from 0.2 mg 293T transfected with V5-tagged Histone H3.1 (N-terminal) cell lysate with HA750086 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750086 at 1/10,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T transfected with V5-tagged Histone H3.1 (N-terminal) cell lysate (input)
Lane 2: HA750086 IP in 293T transfected with V5-tagged Histone H3.1 (N-terminal) cell lysate
Lane 3: Rabbit IgG instead of HA750086 in 293T transfected with V5-tagged Histone H3.1 (N-terminal) cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 2 seconds; ECL: K1801
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"