Phospho-EIF2S1 (S51) Recombinant Rabbit Monoclonal Antibody [SZ01-06] - BSA and Azide free
Catalog# HA750059
Phospho-EIF2S1 (S51) Recombinant Rabbit Monoclonal Antibody [SZ01-06] - BSA and Azide free
-
WB
-
IF-Cell
-
IF-Tissue
-
IHC-P
-
IP
-
FC
-
Human
-
Mouse
-
Rat
-
ET1603-14
含抗保成分
-
unconjugated
概述
产品名称
Phospho-EIF2S1 (S51) Recombinant Rabbit Monoclonal Antibody [SZ01-06] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic phospho-peptide corresponding to residues surrounding Ser51 of Human eIF-2a.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Cell, IF-Tissue, IHC-P, IP, FC
分子量
Predicted band size: 36 kDa
阳性对照
HeLa treated with 50nM Calyculin A for 3 hours whole cell lysate, NIH/3T3 treated with 100nM Calyculin A for 30 minutes whole cell lysate, C6 treated with 100nM Calyculin A for 30 minutes whole cell lysate, THP-1 cell lysate, C2C12 cell lysate, mouse spleen tissue, rat spleen tissue, human liver tissue, human pancreas tissue, mouse brain tissue, mouse placenta tissue, mouse pancreas tissue, human prostate carcinoma tissue, human breast carcinoma tissue, human colon carcinoma tissue, Hela.
偶联
unconjugated
克隆号
SZ01-06
产品特性
形态
Liquid
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:2,000
-
IF-Cell
-
1:50-1:200
-
IF-Tissue
-
1:50-1:200
-
IHC-P
-
1:50-1:500
-
FC
-
1:50-1:100
-
IP
-
Use at an assay dependent concentration.
靶点
功能
Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex. eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B. Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2. This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51.
背景文献
1. Montalbano R et al. Endoplasmic reticulum stress plays a pivotal role in cell death mediated by the pan-deacetylase inhibitor panobinostat in human hepatocellular cancer cells. Transl Oncol 6:143-57 (2013).
2. Kanai R et al. Effect of 34.5 deletions on oncolytic herpes simplex virus activity in brain tumors. J Virol 86:4420-31 (2012).
序列相似性
Belongs to the WD repeat EIF2A family.
组织特异性
Widely expressed. Expressed at higher level in pancreas, heart, brain and placenta.
亚细胞定位
Stress granule, Cytoplasm.
别名
EIF 2 alpha antibody
EIF 2 antibody
EIF 2A antibody
EIF 2alpha antibody
eIF-2-alpha antibody
eIF-2A antibody
EIF-2alpha antibody
EIF2 alpha antibody
EIF2 antibody
EIF2A antibody
展开图片
-
☑ Cell treatment (CT)
Western blot analysis of Phospho-EIF2S1 (S51) on different lysates with Rabbit anti-Phospho-EIF2S1 (S51) antibody (HA750059) at 1/2,000 dilution.
Lane 1: HeLa whole cell lysate (15 µg/Lane)
Lane 2: HeLa treated with 50nM Calyculin A for 3 hours whole cell lysate (15 µg/Lane)
Lane 3: NIH/3T3 whole cell lysate (15 µg/Lane)
Lane 4: NIH/3T3 treated with 100nM Calyculin A for 30 minutes whole cell lysate (15 µg/Lane)
Lane 5: C6 whole cell lysate (20 µg/Lane)
Lane 6: C6 treated with 100nM Calyculin A for 30 minutes whole cell lysate (20 µg/Lane)
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: Lane 1-4: 2 minutes; Lane 5-6: 23 seconds；
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750059) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Western blot analysis of Phospho-EIF2S1 (S51) on THP-1 cell lysates.
Lane 1: THP-1 cells, whole cell lysate, 10ug/lane
Lane 2: THP-1 cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane
All lanes :
Anti-Phospho-EIF2S1 (S51) antibody (HA750059) at 1/500 dilution. Anti-GAPDH antibody (ET1601-4) at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Blocking and diluting buffer: 5% BSA.
Exposure time: 3 minutes 43 seconds -
☑ Cell treatment (CT)
Western blot analysis of Phospho-EIF2S1 (S51) on C2C12 cell lysates.
Lane 1: C2C12 cells, whole cell lysate, 10ug/lane
Lane 2: C2C12 cells treated with 300nM thapsigargin for 30 minutes, whole cell lysates, 10ug/lane
All lanes :
Anti-Phospho-EIF2S1 (S51) antibody (HA750059
) at 1:500 dilution. Anti-GAPDH antibody (ET1601-4) at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Blocking and diluting buffer: 5% BSA.
Exposure time: 3 minutes 43 seconds -
☑ Cell treatment (CT)
Western blot analysis of Phospho-EIF2S1 (S51) on mouse spleen tissue lysates.
Lane 1: mouse spleen tissue, whole tissue lysate, 20ug/lane
Lane 2: mouse spleen tissue treated with 2.8ug/ul lambda-PP for 30 minutes, whole tissue lysates, 20ug/lane
All lanes :
Anti-Phospho-EIF2S1 (S51) antibody (HA750059) at 1/500 dilution. Anti-GAPDH antibody (ET1601-4) at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Blocking and diluting buffer: 5% BSA.
Exposure time: 3 minutes 43 seconds -
☑ Cell treatment (CT)
Western blot analysis of Phospho-EIF2S1 (S51) on rat spleen tissue lysates.
Lane 1: rat spleen tissue, whole tissue lysate, 20ug/lane
Lane 2: rat spleen tissue treated with 2.8ug/ul lambda-PP for 30 minutes, whole tissue lysates, 20ug/lane
All lanes :
Anti-Phospho-EIF2S1 (S51) antibody (HA750059) at 1/500 dilution. Anti-GAPDH antibody (ET1601-4) at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Blocking and diluting buffer: 5% BSA.
Exposure time: 1 minute 15 seconds -
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Phospho-EIF2S1 (S51) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750059, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
-
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Phospho-EIF2S1 (S51) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750059, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
-
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospho-EIF2S1 (S51) antibody (HA750059) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750059) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-Phospho-EIF2S1 (S51) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750059, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
-
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Phospho-EIF2S1 (S51) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750059, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
-
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using anti-Phospho-EIF2S1 (S51) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750059, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
-
☑ Cell treatment (CT),Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Phospho-EIF2S1 (S51) antibody (HA750059) at 1/200 dilution.
A: Untreated human breast carcinoma tissue
B: λ-PPase treated human breast carcinoma tissue
C: Negative control
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750059) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Cell treatment (CT),Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Phospho-EIF2S1 (S51) antibody (HA750059) at 1/200 dilution.
A: Untreated human colon carcinoma tissue
B: λ-PPase treated human colon carcinoma tissue
C: Negative control
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750059) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of Phospho-EIF2S1 (S51) was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (HA750059, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
-
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-Phospho-EIF2S1 (S51) antibody (HA750059) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750059) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
浙公网安备 33019202000643号