LAMP2a Recombinant Rabbit Monoclonal Antibody [SA46-01] - BSA and Azide free
Rmb: 5600
Catalog# HA750016
LAMP2a Recombinant Rabbit Monoclonal Antibody [SA46-01] - BSA and Azide free
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WB
-
IHC-P
-
IP
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Human
-
Mouse
-
Rat
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ET1601-24
含抗保成分
-
unconjugated
概述
产品名称
LAMP2a Recombinant Rabbit Monoclonal Antibody [SA46-01] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human LAMP2a aa 361-410 / 410.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P, IP
靶点分子量
Predicted band size: 45 kDa
阳性对照
SK-MEL-28 cell lysate, HeLa cell lysate, JAR cell lysate, U-937 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse liver tissue lysate, rat liver tissue lysate, rat lung tissue lysate, human kidney tissue, mouse pancreas tissue, rat kidney tissue.
偶联
unconjugated
克隆号
SA46-01
产品特性
形态
Liquid
浓度
存放说明
Store at 2-8℃. Avoid freeze.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
靶点
功能
Lysosome-associated membrane protein 2 (LAMP2), also known as CD107b (Cluster of Differentiation 107b) and Mac-3, is a human gene. Its protein, LAMP2, is one of the lysosome-associated membrane glycoproteins. The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of the gene produces three variants - LAMP-2A, LAMP-2B and LAMP-2C. LAMP-2A is the receptor for chaperone-mediated autophagy. Recently it has been determined that antibodies against LAMP-2 account for a fraction of patients who get a serious kidney disease termed focal necrotizing glomerulonephritis. LAMP-2B is associated with Danon disease.
背景文献
1. Guan, JJ. et al. 2015. DRAM1 regulates apoptosis through increasing protein levels and lysosomal localization of BAX. Cell death & disease. 6: e1624.
2. Gu, G. et al. 2013. Ubiquitin E3 Ligase A20 is Required in Degradation of Microbial Superantigens in Vascular Endothelial Cells. Cell Biochem. Biophys. 66: 649-655.
序列相似性
Belongs to the LAMP family.
组织特异性
Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is detected in spleen, thymus, prostate, testis, small intestine, colon, skeletal muscle, brain, placenta, lung, kidney, ovary and pancreas and liver. Isoform LAMP-2C is detected in small intestine, colon, heart, brain, skeletal muscle, and at lower levels in kidney and placenta.
翻译后修饰
O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans.
亚细胞定位
Cell membrane, Endosome membrane, Lysosome membrane
别名
CD107 antigen-like family member B antibody
CD107b antibody
LAMP 2 antibody
Lamp 2a antibody
LAMP-2 antibody
LAMP2 antibody
LAMP2_HUMAN antibody
Lysosome-associated membrane glycoprotein 2 antibody
Lysosome-associated membrane protein 2 antibody
图片
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This data was developed using ET1601-24, the same antibody clone in a different buffer formulation.
Western blot analysis of LAMP2a on different lysates with Rabbit anti-LAMP2a antibody (ET1601-24) at 1/5,000 dilution.
Lane 1: SK-MEL-28 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: JAR cell lysate (20 µg/Lane)
Lane 4: U-937 cell lysate (20 µg/Lane)
Lane 5: RAW264.7 cell lysate (15 µg/Lane)
Lane 6: NIH/3T3 cell lysate (15 µg/Lane)
Lane 7: PC-12 cell lysate (15 µg/Lane)
Lane 8: Mouse liver tissue lysate (30 µg/Lane)
Lane 9: Rat liver tissue lysate (30 µg/Lane)
Lane 10: Rat lung tissue lysate (30 µg/Lane)
Predicted band size: 45 kDa
Observed band size: 70-140 kDa
Exposure time: Lane 1-4: 2 minutes 37 seconds; Lane 5-10: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-24) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. -
This data was developed using ET1601-24, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-LAMP2a antibody (ET1601-24) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-24) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1601-24, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-LAMP2a antibody (ET1601-24) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-24) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1601-24, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-LAMP2a antibody (ET1601-24) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-24) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1601-24, the same antibody clone in a different buffer formulation.
LAMP2a was immunoprecipitated from 0.2 mg SK-MEL-28 cell lysate with ET1601-24 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1601-24 at 1/2,000 dilution. HRP Conjugated Rabbit IgG kappa light chain antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: SK-MEL-28 cell lysate (Input)
Lane 2: ET1601-24 IP in SK-MEL-28 cell lysate
Lane 3: Rabbit IgG instead of ET1601-24 in SK-MEL-28 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 20 seconds; ECL: K1801
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
LAMP2a Rabbit Polyclonal Antibody
Application: WB,IHC-P
Reactivity: Human,Mouse
Conjugate: unconjugated
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