Biotin Conjugated Human REST Recombinant Rabbit Monoclonal Antibody [PSH21-39] - Detector
Rmb: 1980
概述
产品名称
Biotin Conjugated Human REST Recombinant Rabbit Monoclonal Antibody [PSH21-39] - Detector
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Recombinant Human REST aa 801-1097 (HA211258).
种属反应性
Human
验证应用
ELISA(Det), ELISA
阳性对照
Recombinant Human REST protein (HA211258).
偶联
Biotin
克隆号
PSH21-39
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
ELISA(Det)
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Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH21-38] to Human REST antibody (Capture) (HA725193) and Recombinant Human REST protein (HA211258) as the standard. The reference range value is 78.1-10,000 pg/mL.
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ELISA
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Use at an assay dependent concentration.
靶点
功能
Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, SIN3A and RCOR1, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. Transcriptional repression by REST-CDYL via the recruitment of histone methyltransferase EHMT2 may be important in transformation suppression. Represses the expression of SRRM4 in non-neural cells to prevent the activation of neural-specific splicing events and to prevent production of REST isoform 3. Repressor activity may be inhibited by forming heterodimers with isoform 3, thereby preventing binding to NRSE or binding to corepressors and leading to derepression of target genes. Also maintains repression of neuronal genes in neural stem cells, and allows transcription and differentiation into neurons by dissociation from RE1/NRSE sites of target genes. Thereby is involved in maintaining the quiescent state of adult neural stem cells and preventing premature differentiation into mature neurons.
背景文献
1. Li H. et. al. The deficiency of NRSF/REST enhances the pro-inflammatory function of astrocytes in a model of Parkinson\'s disease. Biochim Biophys Acta Mol Basis Dis. 2020 Jan
2. Zhao Y. et. al. Brain REST/NRSF Is Not Only a Silent Repressor but Also an Active Protector. Mol Neurobiol. 2017 Jan
亚细胞定位
Nucleus, Cytoplasm.
UNIPROT
别名
Neural Restrictive Silencer Factor antibody
Neural-restrictive silencer factor antibody
Neuron Restrictive Silencer Factor antibody
NRSF antibody
RE1 silencing transcription factor antibody
RE1-silencing transcription factor antibody
Repressor binding to the X2 box antibody
rest antibody
REST protein antibody
REST_HUMAN antibody
展开图片
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![Sandwich ELISA analysis of Human REST matched pair antibodies<br /><br />Capture: <a href="/products/HA725193" style="font-weight: bold;text-decoration: underline;">HA725193</a>, Human REST Rabbit mAb [PSH21-38]<br />Detector: <a href="/products/HA725194" style="font-weight: bold;text-decoration: underline;">HA725194</a>, Human REST Rabbit mAb [PSH21-39]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (<a href="/products/HA725193" style="font-weight: bold;text-decoration: underline;">HA725193</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human REST protein (<a href="/products/HA211258" style="font-weight: bold;text-decoration: underline;">HA211258</a>) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725194" style="font-weight: bold;text-decoration: underline;">HA725194</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725194B_1.png?v=20260604174703)
Sandwich ELISA analysis of Human REST matched pair antibodies
Capture: HA725193, Human REST Rabbit mAb [PSH21-38]
Detector: HA725194, Human REST Rabbit mAb [PSH21-39]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725193) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human REST protein (HA211258) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725194, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native REST in Raji extract samples based on a 1,000 µg/ml extract load.<br /><br />Capture: <a href="/products/HA725193" style="font-weight: bold;text-decoration: underline;">HA725193</a>, Human REST Rabbit mAb [PSH21-38]<br />Detector: <a href="/products/HA725194" style="font-weight: bold;text-decoration: underline;">HA725194</a>, Human REST Rabbit mAb [PSH21-39]<br /><br />The concentrations of REST were measured in duplicates, interpolated from the REST standard curve and corrected for sample dilution. Undiluted samples are Raji extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean REST concentration was determined to be 362.650 pg/ml in Raji extract.](https://storage.huabio.cn/huabio/productImg/HA725194B_2.png?v=20260604174703)
Interpolated concentrations of native REST in Raji extract samples based on a 1,000 µg/ml extract load.
Capture: HA725193, Human REST Rabbit mAb [PSH21-38]
Detector: HA725194, Human REST Rabbit mAb [PSH21-39]
The concentrations of REST were measured in duplicates, interpolated from the REST standard curve and corrected for sample dilution. Undiluted samples are Raji extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean REST concentration was determined to be 362.650 pg/ml in Raji extract.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
Human REST Recombinant Rabbit Monoclonal Antibody [PSH21-39] - BSA and Azide free (Detector)
Application: ELISA(Det)
Reactivity: Human
Conjugate: unconjugated
Human REST Recombinant Rabbit Monoclonal Antibody [PSH21-38] - BSA and Azide free (Capture)
Application: ELISA(Cap)
Reactivity: Human
Conjugate: unconjugated
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