Human/Monkey Mannose Receptor(CD206) Recombinant Rabbit Monoclonal Antibody [PSH23-47] - BSA and Azide free (Detector)

Sandwich ELISA analysis of Human Mannose Receptor(CD206) matched pair antibodies

Capture: HA724377, Human/Monkey Mannose Receptor(CD206) Rabbit mAb [PSH23-46]
Detector: HA724378, Human/Monkey Mannose Receptor(CD206) Rabbit mAb [PSH23-47]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724377) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.2% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Mannose Receptor(CD206) protein (HA211390) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA724378, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Mannose Receptor(CD206) in human serum samples.

Capture: HA724377, Human/Monkey Mannose Receptor(CD206) Rabbit mAb [PSH23-46]
Detector: HA724378, Human/Monkey Mannose Receptor(CD206) Rabbit mAb [PSH23-47]

The concentrations of Mannose Receptor(CD206) were measured in duplicates, interpolated from the Mannose Receptor(CD206) standard curve and corrected for sample dilution. Undiluted samples are human serum 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Mannose Receptor(CD206) concentration was determined to be 159.7 ng/ml in human serum.

Interpolated concentrations of native Mannose Receptor(CD206) in Cynomolgus serum samples.

Capture: HA724377, Human/Monkey Mannose Receptor(CD206) Rabbit mAb [PSH23-46]
Detector: HA724378, Human/Monkey Mannose Receptor(CD206) Rabbit mAb [PSH23-47]

The concentrations of Mannose Receptor(CD206) were measured in duplicates, interpolated from the Mannose Receptor(CD206) standard curve and corrected for sample dilution. Undiluted samples are Cynomolgus serum 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Mannose Receptor(CD206) concentration was determined to be 116.1 ng/ml in Cynomolgus serum.

Interpolated concentrations of spiked Mannose Receptor(CD206) in human cell culture media samples.

Capture: HA724377, Human/Monkey Mannose Receptor(CD206) Rabbit mAb [PSH23-46]
Detector: HA724378, Human/Monkey Mannose Receptor(CD206) Rabbit mAb [PSH23-47]

The concentrations of Mannose Receptor(CD206) were measured in duplicates, interpolated from the Mannose Receptor(CD206) standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).