BCMA Recombinant Rabbit Multiclonal Antibody [PSH18-02]
Catalog# HA723967
BCMA Recombinant Rabbit Multiclonal Antibody [PSH18-02]
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IF-Cell
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FC
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Human
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unconjugated
概述
产品名称
BCMA Recombinant Rabbit Multiclonal Antibody [PSH18-02]
抗体类型
Recombinant Rabbit multiclonal
种属反应性
Human
验证应用
IF-Cell, FC
分子量
Predicted band size: 20 kDa
阳性对照
U266.
偶联
unconjugated
克隆号
PSH18-02
反应性数据
| IF-Cell | FC | |
|---|---|---|
| Human |
|
|
| Mouse | ||
| Rat |
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
IF-Cell
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1:100
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FC
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1μg/mL
靶点
功能
B-cell maturation antigen (BCMA or BCM), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17), is a protein that in humans is encoded by the TNFRSF17 gene. TNFRSF17 is a cell surface receptor of the TNF receptor superfamily which recognizes B-cell activating factor (BAFF). Serum B-cell maturation antigen (sBCMA) is the cleaved form of BCMA, found at low levels in the serum of normal patients and generally elevated in patients with multiple myeloma (MM). The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13B/TALL-1/BAFF), and to lead to NF-kappaB and MAPK8/JNK activation. This receptor also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation.
背景文献
1. Sun F et al. Bispecific BCMA/CD24 CAR-T cells control multiple myeloma growth. Nat Commun. 2024 Jan
2. Lee H et al. Mechanisms of antigen escape from BCMA- or GPRC5D-targeted immunotherapies in multiple myeloma. Nat Med. 2023 Sep
亚细胞定位
Cell membrane, Endomembrane system.
UNIPROT
别名
B cell maturation antigen antibody
B cell maturation factor antibody
B cell maturation protein antibody
B-cell maturation protein antibody
BCM antibody
BCMA antibody
CD269 antibody
CD269 antigen antibody
TNFRSF17 antibody
TNR17_HUMAN antibody
展开图片
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☑ Relative expression (RE)
Immunocytochemistry analysis of U266 (positive) and Jurkat (negative) labeling BCMA with Rabbit anti-BCMA antibody (HA723967) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BCMA antibody (HA723967) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
☑ Relative expression (RE)
Flow cytometric analysis of Jurkat (left, negative) and U266 (right, positive) cells labeling BCMA.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA723967, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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