Ly6g Recombinant Rabbit Monoclonal Antibody [PSH14-95]
Catalog# HA723696
Ly6g Recombinant Rabbit Monoclonal Antibody [PSH14-95]
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FC
-
IHC-P
-
Mouse
概述
产品名称
Ly6g Recombinant Rabbit Monoclonal Antibody [PSH14-95]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Mouse Ly6g aa 27-105.
种属反应性
Mouse
验证应用
FC, IHC-P
分子量
Predicted band size: 14 kDa
阳性对照
Mouse bone marrow, C57 mouse blood, mouse spleen tissue, mouse lung tissue.
偶联
unconjugated
克隆号
PSH14-95
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
FC
-
1:1,000
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IHC-P
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1:2,000-1:4,000
靶点
功能
Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells.
背景文献
1. Ruscitti C et al. Recruited atypical Ly6G(+) macrophages license alveolar regeneration after lung injury. Sci Immunol. 2024 Aug
2. Iliakis CS et al. Never trust a single myeloid marker: Ly6G on repair-promoting lung macrophages. Sci Immunol. 2024 Aug
亚细胞定位
Cell membrane.
UNIPROT
别名
Gr-1 antibody
Gr1 antibody
Ly-6G.1 antibody
Ly6G antibody
Lymphocyte antigen 6 complex locus G antibody
Lymphocyte antigen 6G antibody
图片
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Flow cytometric analysis of mouse bone marrow cells labeling Ly6g.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA723696, 1/1,000) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Flow cytometric analysis of C57 mouse blood cells labeling Ly6g.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA723696, 1/1,000) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-Ly6g antibody (HA723696) at 1/4,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723696) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-Ly6g antibody (HA723696) at 1/4,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723696) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"