PD-L1 Recombinant Rabbit Monoclonal Antibody [PSH08-56]
Catalog# HA723002
PD-L1 Recombinant Rabbit Monoclonal Antibody [PSH08-56]
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WB
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IF-Cell
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IHC-P
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IF-Tissue
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Cat
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HA751231
不含抗保成分
-
unconjugated
概述
产品名称
PD-L1 Recombinant Rabbit Monoclonal Antibody [PSH08-56]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within cat PD-L1 aa 1-232.
种属反应性
Cat
验证应用
WB, IF-Cell, IHC-P, IF-Tissue
分子量
Predicted band size: 26 kDa
阳性对照
293T cells transfected with cat PD-L1.
偶联
unconjugated
克隆号
PSH08-56
反应性数据
| WB | IF-Cell | IHC-P | IF-Tissue | |
|---|---|---|---|---|
| Cat |
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:2,000
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IF-Cell
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1:5,000
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IHC-P
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1:20,000
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IF-Tissue
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1:5,000
靶点
功能
Programmed death-ligand 1 (PD-L1) also known as cluster of differentiation 274 (CD274) or B7 homolog 1 (B7-H1) is a protein that in humans is encoded by the CD274 gene. Programmed death-ligand 1 (PD-L1) is a 40kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the adaptive arm of immune systems during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. Normally the adaptive immune system reacts to antigens that are associated with immune system activation by exogenous or endogenous danger signals. In turn, clonal expansion of antigen-specific CD8+ T cells and/or CD4+ helper cells is propagated. The binding of PD-L1 to the inhibitory checkpoint molecule PD-1 transmits an inhibitory signal based on interaction with phosphatases (SHP-1 or SHP-2) via Immunoreceptor Tyrosine-Based Switch Motif (ITSM). This reduces the proliferation of antigen-specific T-cells in lymph nodes, while simultaneously reducing apoptosis in regulatory T cells (anti-inflammatory, suppressive T cells) – further mediated by a lower regulation of the gene Bcl-2. PD-L1 is expressed on both hematopoietic and nonhematopoietic cells in tissues. However, the exact roles of PD-L1 on hematopoietic versus nonhematopoietic cells in modulating immune responses are unclear.
背景文献
1. Zhang YC et al. What role does PDL1 play in EMT changes in tumors and fibrosis? Front Immunol. 2023 Aug
2. Tran-Nguyen VK et al. Structure-based virtual screening for PDL1 dimerizers: Evaluating generic scoring functions. Curr Res Struct Biol. 2022 Jun
亚细胞定位
Membrane.
UNIPROT
别名
B7 H antibody
B7 H1 antibody
B7 homolog 1 antibody
B7-H1 antibody
B7H antibody
B7H1 antibody
CD 274 antibody
CD-274 antibody
CD274 antibody
CD274 antigen antibody
展开图片
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☑ Cell treatment (CT)
Western blot analysis of PD-L1 on different lysates with Rabbit anti-PD-L1 antibody (HA723002) at 1/2,000 dilution.
Lane 1: 293T cell lysate
Lane 2: 293T transfected with cat PD-L1 cell lysate
Predicted band size: 26 kDa
Observed band size: 35/60 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723002) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Immunocytochemistry analysis of 293T cells transfected with or without cat PD-L1 labeling PD-L1 with Rabbit anti-PD-L1 antibody (HA723002) at 1/5,000 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PD-L1 antibody (HA723002) at 1/5,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
☑ Cell treatment (CT)
Immunohistochemical analysis of paraffin-embedded 293T cells transfected with cat PD-L1 with Rabbit anti-PD-L1 antibody (HA723002) at 1/20,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723002) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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