HRP Conjugated 6X His tag Recombinant Rabbit Monoclonal Antibody [PSH07-10]
Rmb: 1500
Catalog# HA722965
HRP Conjugated 6X His tag Recombinant Rabbit Monoclonal Antibody [PSH07-10]
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WB
-
IHC-P
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Species independent
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HRP
概述
产品名称
HRP Conjugated 6X His tag Recombinant Rabbit Monoclonal Antibody [PSH07-10]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide immune sequence is HHHHHHHHC.
种属反应性
Species independent
验证应用
WB, IHC-P
偶联
HRP
克隆号
PSH07-10
反应性数据
| WB | IHC-P | |
|---|---|---|
| Species Independent |
|
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:100,000
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IHC-P
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1:50,000
靶点
功能
His-Tag Antibody detects recombinant proteins containing the 6xHis epitope tag. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
背景文献
暂无
别名
6 His epitope tag antibody
Hexa His tag antibody
HHHHHH epitope tag antibody
HHHHHH tag antibody
His tag antibody
Polyhistidine Tag antibody
图片
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Western blot analysis of 6X His tag on different lysates with Rabbit anti-6X His tag antibody (HA722965) at 1/20,000 dilution.
Lane 1: 293T transfected with His-tagged empty control cell lysate
Lane 2: 293T transfected with His-tagged ACAT2 (N-terminal) cell lysate
Lane 3: 293T transfected with His-tagged Histone H3.1 (C-terminal) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722965) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. -
Immunohistochemical analysis of paraffin-embedded HeLa transfected with His-tagged ACAT2 (N-terminal) cells with Rabbit anti-6X His tag antibody (HA722965) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722965) at 1/10,000 dilution for 1 hour at room temperature. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded HeLa transfected with His-tagged Histon H3.1 (C-terminal) cells with Rabbit anti-6X His tag antibody (HA722965) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722965) at 1/10,000 dilution for 1 hour at room temperature. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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