SLUG Recombinant Rabbit Monoclonal Antibody [PSH07-40]

☑ Relative expression (RE)

Western blot analysis of SLUG on different lysates with Rabbit anti-SLUG antibody (HA722828) at 1/1,000 dilution.

Lane 1: U-87 MG cell lysate
Lane 2: MCF7 cell lysate (negative)
Lane 3: SK-MEL-28 cell lysate
Lane 4: PANC-1 cell lysate (low expression)

Lysates/proteins at 30 µg/Lane.

Predicted band size: 30 kDa
Observed band size: 35 kDa

Exposure time: 2 minutes 7 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722828) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

☑ Relative expression (RE)

Immunocytochemistry analysis of SK-MEL-28 (positive) and PANC-1 (low expression) labeling SLUG with Rabbit anti-SLUG antibody (HA722828) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SLUG antibody (HA722828) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.