SLUG Recombinant Rabbit Monoclonal Antibody [PSH07-40]
Catalog# HA722828
SLUG Recombinant Rabbit Monoclonal Antibody [PSH07-40]
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WB
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IF-Cell
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Human
概述
产品名称
SLUG Recombinant Rabbit Monoclonal Antibody [PSH07-40]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human SLUG aa 1-150.
种属反应性
Human
验证应用
WB, IF-Cell
分子量
Predicted band size: 30 kDa
阳性对照
U-87 MG cell lysate, SK-MEL-28 cell lysate, SK-MEL-28.
偶联
unconjugated
克隆号
PSH07-40
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000
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IF-Cell
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1:100
靶点
功能
This gene encodes a member of the Snail superfamily of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. It regulates differentiation and migration of neural crest cells along with other genes (e.g. FOXD3, SOX9 and SOX10, BMPs) in embryonic life. Mutations in this gene may be associated with sporadic cases of neural tube defects. SNAI2 downregulates expression of E-cadherin in premigratory neural crest cells; thus, SNAI2 induces tightly bound epithelial cells to break into a loose mesenchymal phenotype, allowing gastrulation of mesoderm in the developing embryo. Structurally similar to anti-apoptotic Ces-1 in C. elegans, SLUG is a negative regulator of productive cell death in the developing embryo and adults.
背景文献
1. Li W et al. Deubiquitinase USP20 promotes breast cancer metastasis by stabilizing SNAI2. Genes Dev. 2020 Oct
2. Jin Y et al. SNAI2 promotes the development of ovarian cancer through regulating ferroptosis. Bioengineered. 2022 Mar
亚细胞定位
Nucleus, Cytoplasm.
UNIPROT
别名
MGC10182 antibody
Neural crest transcription factor Slug antibody
Protein snail homolog 2 antibody
Slug (chicken homolog) zinc finger protein antibody
Slug homolog zinc finger protein antibody
Slug zinc finger protein antibody
SLUGH antibody
SLUGH 1 antibody
SLUGH1 antibody
SNAI 2 antibody
展开图片
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☑ Relative expression (RE)
Western blot analysis of SLUG on different lysates with Rabbit anti-SLUG antibody (HA722828) at 1/1,000 dilution.
Lane 1: U-87 MG cell lysate
Lane 2: MCF7 cell lysate (negative)
Lane 3: SK-MEL-28 cell lysate
Lane 4: PANC-1 cell lysate (low expression)
Lysates/proteins at 30 µg/Lane.
Predicted band size: 30 kDa
Observed band size: 35 kDa
Exposure time: 2 minutes 7 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722828) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Relative expression (RE)
Immunocytochemistry analysis of SK-MEL-28 (positive) and PANC-1 (low expression) labeling SLUG with Rabbit anti-SLUG antibody (HA722828) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SLUG antibody (HA722828) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"