Caspase-8 (p18) Recombinant Rabbit Monoclonal Antibody [PSH05-83]
概述
产品名称
Caspase-8 (p18) Recombinant Rabbit Monoclonal Antibody [PSH05-83]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide corresponding to residues surrounding Asp387 of mouse caspase-8 protein.
产品特异性
Caspase-8 (p18) [PSH05-83] Rabbit mAb recognizes endogenous levels of mouse caspase-8 only when cleaved at Asp387. In addition to the p18 subunit, this antibody also detects cleavage products containing the pro-domain with the p18 subunit (p43).
种属反应性
Mouse
验证应用
WB, IF-Cell
分子量
Predicted band size: 55 kDa
阳性对照
RAW264.7 cell lysate, RAW264.7 treated with 1μM Etoposide for 18 hours cell lysate, CTLL-2 cell lysate, RAW264.7 cells treated with 1μM Etoposide for 18 hours.
偶联
unconjugated
克隆号
PSH05-83
反应性数据
| WB | IF-Cell | |
|---|---|---|
| Mouse |
|
|
| Rat |
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:2,000
-
IF-Cell
-
1:100
靶点
功能
Thiol protease that plays a key role in programmed cell death by acting as a molecular switch for apoptosis, necroptosis and pyroptosis, and is required to prevent tissue damage during embryonic development and adulthood. Initiator protease that induces extrinsic apoptosis by mediating cleavage and activation of effector caspases responsible for FAS/CD95-mediated and TNFRSF1A-induced cell death. Cleaves and activates effector caspases CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. Binding to the adapter molecule FADD recruits it to either receptor FAS/CD95 or TNFRSF1A. The resulting aggregate called the death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. In addition to extrinsic apoptosis, also acts as a negative regulator of necroptosis: acts by cleaving RIPK1 at 'Asp-325', which is crucial to inhibit RIPK1 kinase activity, limiting TNF-induced apoptosis, necroptosis and inflammatory response.
背景文献
1. Benchoua A., Couriaud C., Guegan C., Tartier L., Couvert P., Friocourt G., Chelly J., Menissier-de Murcia J., Onteniente B. Active caspase-8 translocates into the nucleus of apoptotic cells to inactivate poly(ADP-ribose) polymerase-2. J. Biol. Chem. 277:34217-34222 (2002)
2. Newton K., Wickliffe K.E., Dugger D.L., Maltzman A., Roose-Girma M., Dohse M., Komuves L., Webster J.D., Dixit V.M. Cleavage of RIPK1 by caspase-8 is crucial for limiting apoptosis and necroptosis. Nature 574:428-431 (2019)
亚细胞定位
Cytoplasm. Nucleus.
UNIPROT
别名
ALPS2B antibody
Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein antibody
Apoptosis related cysteine peptidase antibody
Apoptotic cysteine protease antibody
Apoptotic protease Mch-5 antibody
Apoptotic protease Mch5 antibody
CAP 4 antibody
CAP4 antibody
CASP-8 antibody
CASP8 antibody
展开图片
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☑ Cell treatment (CT)
Western blot analysis of Caspase-8 (p18) on different lysates with Rabbit anti-Caspase-8 (p18) antibody (HA722482) at 1/2,000 dilution.
Lane 1: RAW264.7 cell lysate
Lane 2: RAW264.7 treated with 1μM Etoposide for 18 hours cell lysate
Lane 3: CTLL-2 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 43/18 kDa
Observed band size: 43/18 kDa
Exposure time: 1 minute 20 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722482) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Immunocytochemistry analysis of RAW264.7 cells treated with 1μM Etoposide for 18 hours labeling Caspase-8 (p18) with Rabbit anti-Caspase-8 (p18) antibody (HA722482) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Caspase-8 (p18) antibody (HA722482) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
-
Cyanidin-3-O-Glucoside Mitigates Hepatotoxicity Induced by 2-Amino-3-Methylimidazo[4,5-f]Quinoline via Endogenous and Exogenous Apoptotic Signaling Pathways: Evidence From In Vivo and In Silico Studies
期刊: Molecular Nutrition & Food Research
DOI: 10.1002/mnfr.70363
IF: 4.2
应用: WB
反应种属: Mouse
发表时间: 2025 Dec
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