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Delta-catenin Recombinant Rabbit Monoclonal Antibody [JE75-34]

RMB: 699 特惠 1500

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Catalog# HA722438

Delta-catenin Recombinant Rabbit Monoclonal Antibody [JE75-34]

Application

  • WB

  • IHC-P

  • IP

  • IHC-Fr

Reactivity

  • Human

  • Mouse

  • Rat

概述

产品名称

Delta-catenin Recombinant Rabbit Monoclonal Antibody [JE75-34]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Recombinant protein within Mouse Catenin delta-2 aa 1-100 / 1,247.

种属反应性

Human, Mouse, Rat

验证应用

WB, IHC-P, IP, IHC-Fr

分子量

Predicted band size: 135 kDa

阳性对照

Mouse brain tissue lysate, mouse hippocampus tissue lysate, mouse cerebellum tissue lysate, rat brain tissue lysate, rat hippocampus tissue lysate, rat cerebellum tissue lysate, human brain tissue, mouse brain tissue, rat brain tissue.

偶联

unconjugated

克隆号

JE75-34

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:1,000

  • IHC-P

  • 1:200

  • IP

  • 1-2μg/sample

  • IHC-Fr

  • 1:200

靶点

功能

δ-Catenin is a subfamily of catenin proteins with ten armadillo-repeats and includes the proteins catenin delta-1 and catenin delta-2. Catenin delta-2 is expressed in the brain where it is important for normal cognitive development. Like β-catenin and γ-catenin, δ-catenins seem to interact with presenilins. These catenin-presenilin interaction have implications for cadherin function and regulation of cell-to-cell adhesion. While β-catenin acts as a transcription regulatory protein in the Wnt/TCF pathway, delta-1 catenin has been implicated as a regulator of the NF-κB transcription factor. Palmitoylation of δ-catenin seems to coordinate activity-dependent changes in synaptic adhesion molecules, synapse structure, and receptor localizations that are involved in memory formation.

背景文献

1. Zang X et al. Circular RNA EIF4G3 suppresses gastric cancer progression through inhibition of beta-catenin by promoting delta-catenin ubiquitin degradation and upregulating SIK1. Mol Cancer. 2022 Jul

2. Tan CX et al. delta-Catenin controls astrocyte morphogenesis via layer-specific astrocyte-neuron cadherin interactions. J Cell Biol. 2023 Nov

亚细胞定位

Nucleus, Cell junction, adherens junction, Cell projection, dendrite, Perikaryon.

UNIPROT #

SwissProt: Q9UQB3 Human

SwissProt: O35927 Mouse

SwissProt: O35116 Rat

别名

ARM-repeat protein antibody

Catenin (cadherin associated protein) delta 2 antibody

catenin (cadherin-associated protein), delta 2 (neural plakophilin-related arm-repeat protein) antibody

Catenin delta 2 antibody

Catenin delta-2 antibody

CTND2_HUMAN antibody

CTNND 2 antibody

Ctnnd2 antibody

Delta catenin antibody

Delta-catenin antibody

展开

图片

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Delta-catenin on different lysates with Rabbit anti-Delta-catenin antibody (<a href="/products/HA722438" style="font-weight: bold;text-decoration: underline;">HA722438</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate<br />Lane 2: Mouse hippocampus tissue lysate<br />Lane 3: Mouse heart tissue lysate (negative)<br />Lane 4: Mouse kidney tissue lysate (negative)<br />Lane 5: Mouse cerebellum tissue lysate<br />Lane 6: Rat brain tissue lysate<br />Lane 7: Rat heart tissue lysate (negative)<br />Lane 8: Rat kidney tissue lysate (negative)<br />Lane 9: Rat spleen tissue lysate (negative)<br />Lane 10: Rat hippocampus tissue lysate<br />Lane 11: Rat cerebellum tissue lysate<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 135 kDa<br />Observed band size: 135 kDa<br /><br />Exposure time: 1 minute 2 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722438" style="font-weight: bold;text-decoration: underline;">HA722438</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of Delta-catenin on different lysates with Rabbit anti-Delta-catenin antibody (HA722438) at 1/1,000 dilution.

    Lane 1: Mouse brain tissue lysate
    Lane 2: Mouse hippocampus tissue lysate
    Lane 3: Mouse heart tissue lysate (negative)
    Lane 4: Mouse kidney tissue lysate (negative)
    Lane 5: Mouse cerebellum tissue lysate
    Lane 6: Rat brain tissue lysate
    Lane 7: Rat heart tissue lysate (negative)
    Lane 8: Rat kidney tissue lysate (negative)
    Lane 9: Rat spleen tissue lysate (negative)
    Lane 10: Rat hippocampus tissue lysate
    Lane 11: Rat cerebellum tissue lysate

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 135 kDa
    Observed band size: 135 kDa

    Exposure time: 1 minute 2 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722438) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Delta-catenin was immunoprecipitated from 0.2 mg mouse brain tissue lysate with <a href="/products/HA722438" style="font-weight: bold;text-decoration: underline;">HA722438</a> at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using <a href="/products/HA722438" style="font-weight: bold;text-decoration: underline;">HA722438</a> at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (<a href="/products/NBI01H" style="font-weight: bold;text-decoration: underline;">NBI01H</a>) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: Mouse brain tissue lysate (input)<br />Lane 2: <a href="/products/HA722438" style="font-weight: bold;text-decoration: underline;">HA722438</a> IP in mouse brain tissue lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA722438" style="font-weight: bold;text-decoration: underline;">HA722438</a> in mouse brain tissue lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 17 seconds; ECL: K1801

    Delta-catenin was immunoprecipitated from 0.2 mg mouse brain tissue lysate with HA722438 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722438 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: Mouse brain tissue lysate (input)
    Lane 2: HA722438 IP in mouse brain tissue lysate
    Lane 3: Rabbit IgG instead of HA722438 in mouse brain tissue lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 17 seconds; ECL: K1801

  • Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Delta-catenin antibody (<a href="/products/HA722438" style="font-weight: bold;text-decoration: underline;">HA722438</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722438" style="font-weight: bold;text-decoration: underline;">HA722438</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Delta-catenin antibody (HA722438) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722438) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Delta-catenin antibody (<a href="/products/HA722438" style="font-weight: bold;text-decoration: underline;">HA722438</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722438" style="font-weight: bold;text-decoration: underline;">HA722438</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Delta-catenin antibody (HA722438) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722438) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Delta-catenin antibody (<a href="/products/HA722438" style="font-weight: bold;text-decoration: underline;">HA722438</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722438" style="font-weight: bold;text-decoration: underline;">HA722438</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Delta-catenin antibody (HA722438) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722438) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunofluorescence analysis of frozen rat brain tissue with Rabbit anti-Delta-catenin antibody (<a href="/products/HA722438" style="font-weight: bold;text-decoration: underline;">HA722438</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA722438" style="font-weight: bold;text-decoration: underline;">HA722438</a>, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of frozen rat brain tissue with Rabbit anti-Delta-catenin antibody (HA722438) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722438, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

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