ICAM1 Recombinant Rabbit Monoclonal Antibody [JE77-69]
Rmb: 880 特惠 1500
Catalog# HA722417
ICAM1 Recombinant Rabbit Monoclonal Antibody [JE77-69]
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WB
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IHC-P
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Mouse
-
unconjugated
概述
产品名称
ICAM1 Recombinant Rabbit Monoclonal Antibody [JE77-69]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within rat ICAM1 aa 1-517.
种属反应性
Mouse
验证应用
WB, IHC-P
靶点分子量
Predicted band size: 59 kDa
阳性对照
Mouse kidney tissue lysate, mouse spleen tissue lysate, mouse lung tissue lysate, mouse liver tissue, mouse lung tissue.
偶联
unconjugated
克隆号
JE77-69
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
-
IHC-P
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1:10,000
靶点
功能
This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. Homodimer (Probable). Interacts with MUC1 and promotes cell aggregation in epithelial cells. Interacts with ARHGEF26/SGEF. Interacts (on T cell side) with CD81, CD247 and CD9 at immunological synapses between antigen-presenting cells and T cells.
背景文献
1. Bella J., Kolatkar P.R., Marlor C.W., Greve J.M., Rossmann M.G. The structure of the two amino-terminal domains of human ICAM-1 suggests how it functions as a rhinovirus receptor and as an LFA-1 integrin ligand. Proc. Natl. Acad. Sci. U.S.A. 95:4140-4145 (1998).
2. Shimaoka M., Xiao T., Liu J.H., Yang Y., Dong Y., Jun C.D., McCormack A., Zhang R., Joachimiak A., Springer T.A. Structures of the alpha L I domain and its complex with ICAM-1 reveal a shape-shifting pathway for integrin regulation. Cell 112:99-111 (2003)
亚细胞定位
Membrane.
UNIPROT
别名
Antigen identified by monoclonal antibody BB2 antibody
BB 2 antibody
BB2 antibody
CD 54 antibody
CD_antigen=CD54 antibody
CD54 antibody
Cell surface glycoprotein P3.58 antibody
Human rhinovirus receptor antibody
ICAM 1 antibody
ICAM-1 antibody
展开图片
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Western blot analysis of ICAM1 on different lysates with Rabbit anti-ICAM1 antibody (HA722417) at 1/1,000 dilution.
Lane 1: Mouse kidney tissue lysate
Lane 2: Mouse spleen tissue lysate
Lane 3: Mouse lung tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 59 kDa
Observed band size: 100 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722417) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-ICAM1 antibody (HA722417) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722417) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-ICAM1 antibody (HA722417) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722417) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
引文
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Huangkui capsule, an extract from Abelmoschus manihot (L.) medic, inhibits adrenal aldosterone synthesis and renal ERK/EGR1 pathway in the treatment of diabetic kidney disease
期刊: Journal Of Ethnopharmacology
DOI: 10.1016/j.jep.2025.119936
IF: 4.8
应用: WB
反应种属: Mouse
发表时间: 2025 May
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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