NCOA4 Recombinant Rabbit Monoclonal Antibody [PSH05-44]
Catalog# HA722282
NCOA4 Recombinant Rabbit Monoclonal Antibody [PSH05-44]
-
WB
-
Human
概述
产品名称
NCOA4 Recombinant Rabbit Monoclonal Antibody [PSH05-44]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human NCOA4 aa 1-400 / 614.
种属反应性
Human
验证应用
WB
分子量
Predicted band size: 70 kDa
阳性对照
HeLa cell lysate, A-172 cell lysate, A549 cell lysate, A431 cell lysate, HL-60 cell lysate, NIH:OVCAR-3 cell lysate, A375 cell lysate.
偶联
unconjugated
克隆号
PSH05-44
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000-1:5,000
靶点
功能
Nuclear receptor coactivator 4, also known as Androgen Receptor Activator (ARA70), is a protein that in humans is encoded by the NCOA4 gene. It plays an important role in ferritinophagy, acting as a cargo receptor, binding to the ferritin heavy chain and latching on to ATG8 on the surface of the autophagosome.
背景文献
1. Santana-Codina N et al. The Role of NCOA4-Mediated Ferritinophagy in Ferroptosis. Adv Exp Med Biol. 2021
2. Wu H et al. ATM orchestrates ferritinophagy and ferroptosis by phosphorylating NCOA4. Autophagy. 2023 Jul
亚细胞定位
Nucleus.
UNIPROT
别名
70 kDa androgen receptor activator antibody
70 kDa androgen receptor coactivator antibody
70 kDa AR activator antibody
70 kDa AR-activator antibody
Androgen receptor coactivator 70 kD antibody
Androgen receptor coactivator 70 kDa protein antibody
Androgen receptor-associated protein of 70 kDa antibody
ARA 70 antibody
ARA70 antibody
DKFZp762E1112 antibody
展开图片
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Western blot analysis of NCOA4 on different lysates with Rabbit anti-NCOA4 antibody (HA722282) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: A-172 cell lysate
Lane 3: A549 cell lysate
Lane 4: A431 cell lysate
Lane 5: HL-60 cell lysate
Lane 6: NIH:OVCAR-3 cell lysate
Lane 7: A375 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 70 kDa
Observed band size: 75 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722282) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of NCOA4 on different lysates with Rabbit anti-NCOA4 antibody (HA722282) at 1/5,000 dilution.
Lane 1: KYSE150-si NT cell lysate
Lane 2: KYSE150-si NCOA4 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 70 kDa
Observed band size: 75 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722282) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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