NCOA4 Recombinant Rabbit Monoclonal Antibody [PSH05-45]
概述
产品名称
NCOA4 Recombinant Rabbit Monoclonal Antibody [PSH05-45]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human NCOA4 aa 1-400 / 614.
种属反应性
Human, Mouse, Rat
验证应用
IHC-P, IF-Tissue
分子量
Predicted band size: 70 kDa
阳性对照
Human kidney tissue, human prostate cancer tissue, mouse colon tissue, rat colon tissue.
偶联
unconjugated
克隆号
PSH05-45
反应性数据
| IHC-P | IF-Tissue | |
|---|---|---|
| Human |
|
|
| Mouse |
|
|
| Rat |
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
IHC-P
-
1:500-1:1,500
-
IF-Tissue
-
1:200
靶点
功能
Nuclear receptor coactivator 4, also known as Androgen Receptor Activator (ARA70), is a protein that in humans is encoded by the NCOA4 gene. It plays an important role in ferritinophagy, acting as a cargo receptor, binding to the ferritin heavy chain and latching on to ATG8 on the surface of the autophagosome.
背景文献
1. Santana-Codina N et al. The Role of NCOA4-Mediated Ferritinophagy in Ferroptosis. Adv Exp Med Biol. 2021
2. Wu H et al. ATM orchestrates ferritinophagy and ferroptosis by phosphorylating NCOA4. Autophagy. 2023 Jul
亚细胞定位
Nucleus.
别名
70 kDa androgen receptor activator antibody
70 kDa androgen receptor coactivator antibody
70 kDa AR activator antibody
70 kDa AR-activator antibody
Androgen receptor coactivator 70 kD antibody
Androgen receptor coactivator 70 kDa protein antibody
Androgen receptor-associated protein of 70 kDa antibody
ARA 70 antibody
ARA70 antibody
DKFZp762E1112 antibody
展开图片
-
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-NCOA4 antibody (HA722283) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722283) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-NCOA4 antibody (HA722283) at 1/1,500 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722283) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-NCOA4 antibody (HA722283) at 1/1,500 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722283) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-NCOA4 antibody (HA722283) at 1/1,500 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722283) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
-
Low-Dose Valproic Acid Ameliorates Osteoporosis by Restoring Redox Homeostasis and Suppressing Ferritinophagy-Dependent Ferroptosis via EZH2/H3K27me3 Signaling
期刊: Free Radical Biology And Medicine
DOI: 10.1016/j.freeradbiomed.2025.09.023
IF: 8.2
应用: IHC
反应种属: Rat
发表时间: 2025 Sept
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