CCT2 Recombinant Rabbit Monoclonal Antibody [JE37-96]
Catalog# HA722240
CCT2 Recombinant Rabbit Monoclonal Antibody [JE37-96]
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WB
-
IP
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Human
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Mouse
-
Rat
概述
产品名称
CCT2 Recombinant Rabbit Monoclonal Antibody [JE37-96]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human CCT2 aa 500-535.
种属反应性
Human, Mouse, Rat
验证应用
WB, IP
分子量
Predicted band size: 57 kDa
阳性对照
HeLa cell lysate, NIH/3T3 cell lysate, C6 cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate.
偶联
unconjugated
克隆号
JE37-96
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
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IP
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1-2μg/sample
靶点
功能
T-complex protein 1 subunit beta is a protein that in humans is encoded by the CCT2 gene. This gene encodes a molecular chaperone that is member of the TRiC complex. This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of the gene described in this record have been observed but have not been thoroughly characterized. CCT2 (gene) has been shown to interact with PPP4C.
背景文献
1. Ma X et al. CCT2 is an aggrephagy receptor for clearance of solid protein aggregates. Cell. 2022 Apr
2. Zhang Z et al. CCT2, a newly identified aggrephagy receptor in mammals, specifically mediates the autophagic clearance of solid protein aggregates. Autophagy. 2022 Jul
亚细胞定位
Cytoplasm.
别名
99D8.1 antibody
CCT 2 antibody
CCT beta antibody
CCT-beta antibody
CCT2 antibody
CCTB antibody
Chaperonin containing t complex polypeptide 1 beta subunit antibody
Chaperonin containing t complex polypeptide 1 subunit 2 antibody
Chaperonin containing TCP1 subunit 2 antibody
Chaperonin containing TCP1 subunit 2 (beta) antibody
展开图片
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Western blot analysis of CCT2 on different lysates with Rabbit anti-CCT2 antibody (HA722240) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: NIH/3T3 cell lysate (20 µg/Lane)
Lane 3: C6 cell lysate (20 µg/Lane)
Lane 4: Human brain tissue lysate (40 µg/Lane)
Lane 5: Mouse brain tissue lysate (40 µg/Lane)
Lane 6: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 57 kDa
Observed band size: 50 kDa
Exposure time: 21 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722240) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
CCT2 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA722240 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722240 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: HA722240 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of HA722240 in HeLa cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 5 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"