MDH1 Recombinant Rabbit Monoclonal Antibody [JE35-03]
Catalog# HA722196
MDH1 Recombinant Rabbit Monoclonal Antibody [JE35-03]
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WB
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IF-Cell
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FC
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Human
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Mouse
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Rat
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unconjugated
概述
产品名称
MDH1 Recombinant Rabbit Monoclonal Antibody [JE35-03]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human MDH1 aa 285-334 / 334.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Cell, FC
分子量
Predicted band size: 36 kDa
阳性对照
Jurkat cell lysate, HepG2 cell lysate, U-937 cell lysate, HL-60 cell lysate, NIH/3T3 cell lysate, rat kidney tissue lysate, U-937, NIH/3T3.
偶联
unconjugated
克隆号
JE35-03
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000
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IF-Cell
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1:100
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FC
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1:1,000
靶点
功能
Malate dehydrogenase, cytoplasmic also known as malate dehydrogenase 1 is an enzyme that in humans is encoded by the MDH1 gene. Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
背景文献
1. Hu M et al. MDH1 and MDH2 Promote Cell Viability of Primary AT2 Cells by Increasing Glucose Uptake. Comput Math Methods Med. 2022 Sep
2. Wang M et al. Upregulation of MDH1 acetylation by HDAC6 inhibition protects against oxidative stress-derived neuronal apoptosis following intracerebral hemorrhage. Cell Mol Life Sci. 2022 Jun
亚细胞定位
Cytoplasm, cytosol.
别名
cytoplasmic antibody
Cytosolic malate dehydrogenase antibody
Diiodophenylpyruvate reductase antibody
Malate dehydrogenase 1, NAD (soluble) antibody
Malate dehydrogenase antibody
Malate dehydrogenase cytoplasmic antibody
MDH s antibody
mdh1 antibody
MDHA antibody
MDHC_HUMAN antibody
展开图片
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Western blot analysis of MDH1 on different lysates with Rabbit anti-MDH1 antibody (HA722196) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: U-937 cell lysate (20 µg/Lane)
Lane 4: HL-60 cell lysate (20 µg/Lane)
Lane 5: NIH/3T3 cell lysate (20 µg/Lane)
Lane 6: Rat kidney tissue lysate (40 µg/Lane)
Predicted band size: 36 kDa
Observed band size: 35 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722196) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of U-937 cells labeling MDH1 with Rabbit anti-MDH1 antibody (HA722196) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MDH1 antibody (HA722196) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of U-937 cells labeling MDH1.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA722196, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Flow cytometric analysis of NIH/3T3 cells labeling MDH1.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA722196, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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