p38 MAPK Recombinant Rabbit Multiclonal Antibody [PSH03-72]
概述
产品名称
p38 MAPK Recombinant Rabbit Multiclonal Antibody [PSH03-72]
抗体类型
Recombinant Rabbit multiclonal
免疫原
Recombinant protein within human MAPK12 aa 1-367 / 367.
产品特异性
Anti-p38 MAPK Antibody [PSH03-72] detects endogenous levels of total p38α, p38β, p38γ and p38δ MAPK protein.
种属反应性
Human, Mouse, Rat, Monkey
验证应用
WB
分子量
Predicted band size: 42 kDa
阳性对照
293T cell lysate, HeLa cell lysate, K-562 cell lysate, COS-1 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate.
偶联
unconjugated
克隆号
PSH03-72
RRID
反应性数据
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
| Monkey |
|
| Pig |
|
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
靶点
功能
A mitogen-activated protein kinase (MAPK or MAP kinase) is a type of protein kinase that is specific to the amino acids serine and threonine (i.e., a serine/threonine-specific protein kinase). MAPKs are involved in directing cellular responses to a diverse array of stimuli, such as mitogens, osmotic stress, heat shock and proinflammatory cytokines. They regulate cell functions including proliferation, gene expression, differentiation, mitosis, cell survival, and apoptosis. MAP kinases are found in eukaryotes only, but they are fairly diverse and encountered in all animals, fungi and plants, and even in an array of unicellular eukaryotes. MAPKs belong to the CMGC (CDK/MAPK/GSK3/CLK) kinase group. The closest relatives of MAPKs are the cyclin-dependent kinases (CDKs). p38 MAP Kinase Antibody detects endogenous levels of total p38 alpha, p38 beta, p38 gamma, p38 delta protein.
背景文献
1. Wang J et al. Pan-cancer analysis supports MAPK12 as a potential prognostic and immunotherapeutic target in multiple tumor types, including in THCA. Oncol Lett. 2022 Oct
2. Yang R et al. Prolactin Regulates Ovine Ovarian Granulosa Cell Apoptosis by Affecting the Expression of MAPK12 Gene. Int J Mol Sci. 2023 Jun
亚细胞定位
Cytoplasm, Nucleus, Mitochondrion.
UNIPROT
别名
ERK 6 antibody
ERK-6 antibody
ERK6 antibody
Extracellular signal-regulated kinase 6 antibody
MAP kinase 12 antibody
MAP kinase p38 gamma antibody
MAPK 12 antibody
Mapk12 antibody
Mitogen Activated Protein Kinase 12 antibody
Mitogen activated protein kinase p38 gamma antibody
展开图片
-
Western blot analysis of p38 MAPK on different lysates with Rabbit anti-p38 MAPK antibody (HA722036) at 1/1,000 dilution.
Lane 1: 293T cell lysate
Lane 2: HeLa cell lysate
Lane 3: K-562 cell lysate
Lane 4: COS-1 cell lysate
Lane 5: Neuro-2a cell lysate
Lane 6: PC-12 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 4 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722036) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
-
TGM2 regulated by transcription factor NR3C1 drives p38 MAPK-mediated tumor progression and immune evasion in lung squamous cell carcinoma
期刊: Frontiers In Immunology
DOI: 10.3389/fimmu.2025.1595907
IF: 5.9
应用: WB
反应种属: Human
发表时间: 2025 Sept
-
Exopolysaccharides from Enterococcus faecium WH strain inhibit porcine epidemic diarrhea virus infection in vitro
期刊: Veterinary Microbiology
DOI: 10.1016/j.vetmic.2025.110821
IF: 2.7
应用: WB
反应种属: Pig
发表时间: 2025 Nov
-
Selenomethionine activates MAPK signaling to activate epidermal keratinocyte migration, speeding skin wound healing
期刊: Biochemical And Biophysical Research Communications
DOI: 10.1016/j.bbrc.2025.152398
IF: 2.2
应用: WB
反应种属: Human
发表时间: 2025 Jul
-
SH2B1 Promotes Apoptosis in Diabetic Cataract via p38 MAPK Pathway
期刊: iScience
DOI: 10.1016/j.isci.2024.111735
IF: 4.1
应用: WB
反应种属: Human
发表时间: 2025 Jan
-
SH2B1 Promotes Apoptosis in Diabetic Cataract via p38 MAPK Pathway
期刊: iScience
DOI:
IF: 4.6
应用: WB
反应种属: Human
发表时间: 2025 Jan
-
The anti-inflammatory effects of paeonol from Cynanchum paniculatum extracts on LPS-induced macrophage RAW 264.7 cells and Helicobacter pylori-infected gastrointestinal mucosal damaged zebrafish
期刊: Journal Of Ethnopharmacology
DOI: 10.1016/j.jep.2025.119530
IF: 4.8
应用: WB
反应种属: Mouse
发表时间: 2025 Feb
-
Rv2741 Promotes Mycobacterium Survival by Modulating Macrophage Function via the IL-1α-MAPK Axis
期刊: ACS Infectious Diseases
DOI: 10.1021/acsinfecdis.4c00790
IF: 4
应用: WB
反应种属: Mouse
发表时间: 2025 Feb
-
Jiawei Taohe Chengqi Decoction attenuates CCl4 induced hepatic fibrosis by inhibiting HSCs activation via TGF-β1/CUGBP1 and IFN-γ/Smad7 pathway
期刊: Phytomedicine
DOI: 10.1016/j.phymed.2024.155916
IF: 6.7
应用: WB
反应种属: Mouse,Human
发表时间: 2024 Jul
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