TRADD Recombinant Rabbit Monoclonal Antibody [JE32-60]
Catalog# HA722000
TRADD Recombinant Rabbit Monoclonal Antibody [JE32-60]
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WB
-
IF-Cell
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Human
-
Mouse
-
Rat
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unconjugated
概述
产品名称
TRADD Recombinant Rabbit Monoclonal Antibody [JE32-60]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human TRADD aa 163-312 / 312.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Cell
分子量
Predicted band size: 34 kDa
阳性对照
HeLa cell lysate, Jurkat cell lysate, RAW264.7 cell lysate, C6 cell lysate, PC-12 cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, HL-60 cell lysate, K-562 cell lysate, Ramos cell lysate, C2C12 cell lysate, MCF7.
偶联
unconjugated
克隆号
JE32-60
RRID
反应性数据
| WB | IF-Cell | |
|---|---|---|
| Human |
|
|
| Mouse |
|
|
| Rat |
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
-
IF-Cell
-
1:100
靶点
功能
Tumor necrosis factor receptor type 1-associated DEATH domain protein is a protein that in humans is encoded by the TRADD gene. TRADD is an adaptor protein. The protein encoded by this gene is a death domain containing adaptor molecule that interacts with TNFRSF1A/TNFR1 and mediates programmed cell death signaling and NF-κB activation. This protein binds adaptor protein TRAF2, reduces the recruitment of inhibitor-of-apoptosis proteins (IAPs) by TRAF2, and thus suppresses TRAF2 mediated apoptosis. This protein can also interact with receptor TNFRSF6/FAS and adaptor protein FADD/MORT1, and is involved in the Fas-induced cell death pathway.
背景文献
1. Xu D et al. Modulating TRADD to restore cellular homeostasis and inhibit apoptosis. Nature. 2020 Nov
2. Feoktistova M et al. RIPK1 and TRADD Regulate TNF-Induced Signaling and Ripoptosome Formation. Int J Mol Sci. 2021 Nov
亚细胞定位
Nucleus, Cytoplasm, cytoskeleton.
别名
9130005N23Rik antibody
AA930854 antibody
TNFR1 associated DEATH domain protein antibody
TNFR1-associated DEATH domain protein antibody
TNFRSF1A associated via death domain antibody
TNFRSF1A-associated via death domain antibody
tradd antibody
TRADD_HUMAN antibody
Tumor necrosis factor receptor type 1 associated DEATH domain protein antibody
Tumor necrosis factor receptor type 1-associated DEATH domain protein antibody
图片
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Western blot analysis of TRADD on different lysates with Rabbit anti-TRADD antibody (HA722000) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: RAW264.7 cell lysate (20 µg/Lane)
Lane 4: C6 cell lysate (20 µg/Lane)
Lane 5: PC-12 cell lysate (20 µg/Lane)
Lane 6: Mouse spleen tissue lysate (40 µg/Lane)
Lane 7: Rat spleen tissue lysate (40 µg/Lane)
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 1 minute 50 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722000) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of TRADD on different lysates with Rabbit anti-TRADD antibody (HA722000) at 1/1,000 dilution.
Lane 1: HL-60 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: K-562 cell lysate (20 µg/Lane)
Lane 4: Ramos cell lysate (20 µg/Lane)
Lane 5: Jurkat cell lysate (20 µg/Lane)
Lane 6: C2C12 cell lysate (20 µg/Lane)
Lane 7: PC-12 cell lysate (20 µg/Lane)
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 2 minutes 18 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722000) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of MCF7 cells labeling TRADD with Rabbit anti-TRADD antibody (HA722000) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TRADD antibody (HA722000) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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