NDUFV2 Recombinant Rabbit Monoclonal Antibody [PSH02-86]
Catalog# HA721869
NDUFV2 Recombinant Rabbit Monoclonal Antibody [PSH02-86]
-
WB
-
IHC-P
-
IF-Cell
-
Human
-
Mouse
-
Rat
概述
产品名称
NDUFV2 Recombinant Rabbit Monoclonal Antibody [PSH02-86]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human NDUFV2 aa 1-249 / 249.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P, IF-Cell
分子量
Predicted band size: 27 kDa
阳性对照
A549 cell lysate, HeLa cell lysate, Jurkat cell lysate, Ramos cell lysate, Raji cell lysate, K-562 cell lysate, A431 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, mouse heart tissue lysate, rat heart tissue lysate, human kidney tissue lysate, mouse kidney tissue lysate, RAW264.7, human heart tissue, human kidney tissue, mouse heart tissue, mouse kidney tissue, rat heart tissue, rat kidney tissue.
偶联
unconjugated
克隆号
PSH02-86
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:2,000
-
IHC-P
-
1:2,000-1:5,000
-
IF-Cell
-
1:2,000
靶点
功能
The NADH-ubiquinone oxidoreductase complex (complex I) of the mitochondrial respiratory chain catalyzes the transfer of electrons from NADH to ubiquinone, and consists of at least 43 subunits. The complex is located in the inner mitochondrial membrane. This gene encodes the 24 kDa subunit of complex I, and is involved in electron transfer. Mutations in this gene are implicated in Parkinson's disease, bipolar disorder, schizophrenia, and have been found in one case of early onset hypertrophic cardiomyopathy and encephalopathy. A non-transcribed pseudogene of this locus is found on chromosome 19.
背景文献
1. Pamplona R et al. Is the NDUFV2 subunit of the hydrophilic complex I domain a key determinant of animal longevity? FEBS J. 2021 Dec
2. Chella Krishnan K et al. Sex-specific genetic regulation of adipose mitochondria and metabolic syndrome by Ndufv2. Nat Metab. 2021 Nov
亚细胞定位
Mitochondrion inner membrane.
别名
24kDa subunit of Complex I antibody
CI-24k antibody
complex I 24kDa subunit antibody
complex I, mitochondrial respiratory 2 antibody
mitochondrial antibody
NADH dehydrogenase (ubiquinone) flavoprotein 2 antibody
NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa antibody
NADH dehydrogenase [ubiquinone] flavoprotein 2 antibody
NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial antibody
NADH ubiquinone oxidoreductase 24 kDa subunit antibody
展开图片
-
Western blot analysis of NDUFV2 on different lysates with Rabbit anti-NDUFV2 antibody (HA721869) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution.
Lane 1: A549 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: Jurkat cell lysate (20 µg/Lane)
Lane 4: Ramos cell lysate (20 µg/Lane)
Lane 5: Raji cell lysate (20 µg/Lane)
Lane 6: K-562 cell lysate (20 µg/Lane)
Lane 7: A431 cell lysate (20 µg/Lane)
Lane 8: NIH/3T3 cell lysate (20 µg/Lane)
Lane 9: RAW264.7 cell lysate (20 µg/Lane)
Lane 10: PC-12 cell lysate (20 µg/Lane)
Lane 11: Mouse heart tissue lysate (40 µg/Lane)
Lane 12: Rat heart tissue lysate (40 µg/Lane)
Lane 13: Human kidney tissue lysate (40 µg/Lane)
Lane 14: Mouse kidney tissue lysate (40 µg/Lane)
Predicted band size: 27 kDa
Observed band size: 24 kDa
Exposure time: 1 minute 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721869) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of RAW264.7 cells labeling NDUFV2 with Rabbit anti-NDUFV2 antibody (HA721869) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NDUFV2 antibody (HA721869) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. -
Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-NDUFV2 antibody (HA721869) at 1/2,000 dilution and competitor's antibody at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721869) at 1/2,000 dilution and competitor's antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-NDUFV2 antibody (HA721869) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721869) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-NDUFV2 antibody (HA721869) at 1/2,000 dilution and competitor's antibody at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721869) at 1/2,000 dilution and competitor's antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-NDUFV2 antibody (HA721869) at 1/2,000 dilution and competitor's antibody at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721869) at 1/2,000 dilution and competitor's antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-NDUFV2 antibody (HA721869) at 1/2,000 dilution and competitor's antibody at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721869) at 1/2,000 dilution and competitor's antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-NDUFV2 antibody (HA721869) at 1/2,000 dilution and competitor's antibody at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721869) at 1/2,000 dilution and competitor's antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Knockdown (KD)
Western blot analysis of NDUFV2 on different lysates with Rabbit anti-NDUFV2 antibody (HA721869) at 1/2,000 dilution.
Lane 1: A549-si NT cell lysate
Lane 2: A549-si NDUFV2 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 27 kDa
Observed band size: 24 kDa
Exposure time: 16 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721869) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
NDUFV2 Recombinant Rabbit Monoclonal Antibody [PSH02-86] - BSA and Azide free
Application: WB,IHC-P,IF-Cell
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
