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beta Amyloid 1-40 Recombinant Rabbit Monoclonal Antibody [PSH02-82]

RMB: 699 特惠 1500

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Catalog# HA721788

beta Amyloid 1-40 Recombinant Rabbit Monoclonal Antibody [PSH02-82]

Application

  • IHC-P

  • WB

Reactivity

  • Human

  • Mouse

概述

产品名称

beta Amyloid 1-40 Recombinant Rabbit Monoclonal Antibody [PSH02-82]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within Human beta Amyloid 1-40 peptide.

种属反应性

Human, Mouse

验证应用

IHC-P, WB

分子量

Predicted band size: 4 kDa

阳性对照

APP/PS1, 6-month mouse of AD brain tissue.

偶联

unconjugated

克隆号

PSH02-82

RRID

AB_3072900

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • IHC-P

  • 1:1,000

  • WB

  • 1:1,000

靶点

功能

This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene.

背景文献

1. Janelidze S et al. Head-to-Head Comparison of 8 Plasma Amyloid-β 42/40 Assays in Alzheimer Disease. JAMA Neurol. 2021 Nov

亚细胞定位

Cell membrane, Membrane, Perikaryon, Cell projection, growth cone, Membrane, clathrin-coated pit, Early endosome, Cytoplasmic vesicle.

UNIPROT #

SwissProt: P05067 Human

SwissProt: P12023 Mouse

别名

A4 antibody

A4_HUMAN antibody

AAA antibody

ABETA antibody

ABPP antibody

AICD-50 antibody

AICD-57 antibody

AICD-59 antibody

AID(50) antibody

AID(57) antibody

展开

图片

  • Western blot analysis of beta Amyloid 1-40 on different peptides with Rabbit anti-beta Amyloid 1-40 antibody (<a href="/products/HA721788" style="font-weight: bold;text-decoration: underline;">HA721788</a>) at 1/1,000 dilution.<br /><br />Lane 1: Human Aβ37 full length peptide (negative)<br />Lane 2: Human Aβ38 full length peptide (negative)<br />Lane 3: Human Aβ39 full length peptide (negative)<br />Lane 4: Human Aβ40 full length peptide (positive)<br />Lane 5: Human Aβ41 full length peptide (negative)<br />Lane 6: Human Aβ42 full length peptide (negative)<br />Lane 7: Human Aβ43 full length peptide (negative)<br />Lane 8: Mouse Aβ40 full length peptide (positive)<br /><br />Lysates/proteins at 100 ng/Lane.<br /><br />Predicted band size: 4 kDa<br />Observed band size: 4 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721788" style="font-weight: bold;text-decoration: underline;">HA721788</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of beta Amyloid 1-40 on different peptides with Rabbit anti-beta Amyloid 1-40 antibody (HA721788) at 1/1,000 dilution.

    Lane 1: Human Aβ37 full length peptide (negative)
    Lane 2: Human Aβ38 full length peptide (negative)
    Lane 3: Human Aβ39 full length peptide (negative)
    Lane 4: Human Aβ40 full length peptide (positive)
    Lane 5: Human Aβ41 full length peptide (negative)
    Lane 6: Human Aβ42 full length peptide (negative)
    Lane 7: Human Aβ43 full length peptide (negative)
    Lane 8: Mouse Aβ40 full length peptide (positive)

    Lysates/proteins at 100 ng/Lane.

    Predicted band size: 4 kDa
    Observed band size: 4 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721788) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded APP/PS1, 6-month mouse of AD brain tissue with Rabbit anti-beta Amyloid 1-40 antibody (<a href="/products/HA721788" style="font-weight: bold;text-decoration: underline;">HA721788</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721788" style="font-weight: bold;text-decoration: underline;">HA721788</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded APP/PS1, 6-month mouse of AD brain tissue with Rabbit anti-beta Amyloid 1-40 antibody (HA721788) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721788) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded mouse brain tissue (negative control) with Rabbit anti-beta Amyloid 1-40 antibody (<a href="/products/HA721788" style="font-weight: bold;text-decoration: underline;">HA721788</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721788" style="font-weight: bold;text-decoration: underline;">HA721788</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue (negative control) with Rabbit anti-beta Amyloid 1-40 antibody (HA721788) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721788) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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