Thyroid Hormone Receptor alpha Recombinant Rabbit Monoclonal Antibody [JE40-49]
Catalog# HA721663
Thyroid Hormone Receptor alpha Recombinant Rabbit Monoclonal Antibody [JE40-49]
-
WB
-
Human
-
unconjugated
概述
产品名称
Thyroid Hormone Receptor alpha Recombinant Rabbit Monoclonal Antibody [JE40-49]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human Thyroid Hormone Receptor alpha aa 416-465 / 490.
种属反应性
Human
验证应用
WB
分子量
Predicted band size: 55 kDa
阳性对照
A431 cell lysate, SW480 cell lysate.
偶联
unconjugated
克隆号
JE40-49
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
靶点
功能
Thyroid hormone receptor alpha (TR-alpha) also known as nuclear receptor subfamily 1, group A, member 1 (NR1A1), is a nuclear receptor protein that in humans is encoded by the THRA gene. The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Alternatively spliced transcript variants encoding distinct isoforms have been reported. Mutations of the THRA gene may cause nongoitrous congenital hypothyroidism-6, a subtype of congenital hypothyroidism.
背景文献
1. Liu S et al. Triiodothyronine (T3) promotes brown fat hyperplasia via thyroid hormone receptor α mediated adipocyte progenitor cell proliferation. Nat Commun. 2022 Jun
2. Han CR et al. Thyroid Hormone Receptor α Mutations Cause Heart Defects in Zebrafish. Thyroid. 2021 Feb
亚细胞定位
Nucleus; Cytoplasm, Nucleus.
UNIPROT
别名
AR7 antibody
c-erbA-1 antibody
C-erbA-alpha antibody
CHNG6 antibody
EAR-7 antibody
EAR7 antibody
ERB-T-1 antibody
ERBA antibody
ERBA-related 7 antibody
ERBA1 antibody
展开图片
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Western blot analysis of Thyroid Hormone Receptor alpha on different lysates with Rabbit anti-Thyroid Hormone Receptor alpha antibody (HA721663) at 1/1,000 dilution.
Lane 1: A431 cell lysate
Lane 2: SW480 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721663) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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